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- Clone
- W23019A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD116, Colony Stimulating Factor 2 Receptor Alpha Subunit, Csfgmra, GM-CSFR, GM-CSF-Ra
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant mouse GM-CSF (Cat. No. 576306) (black circles) binds to immobilized mouse GM-CSFRa (Cat. No. 771106). Ultra-LEAF™ purified anti-mouse CD116 (GM-CSF R alpha) (clone W23019A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2a, κ Isotype Control (Cat. No. 400544) (gray triangles) does not have this effect. This antibody blocks the binding of mouse GM-CSF to 2 µg/mL immobilized recombinant mouse GM-CSFRα with ND50 range: 0.04-0.24 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 642453 | 100 µg | £305 | ||||
| 642454 | 1 mg | £702 | ||||
GM-CSF was initially isolated from mouse lung cell-conditioned media as a small glycoprotein which is able to generate both macrophage and granulocyte colonies from mouse bone marrow precursor cells. GM-CSF binds specifically to its heterodimer receptor, composed of a cytokine-specific α-chain (GM-CSF receptor α chain, GMRα) and β-chain (βc), which is shared with the receptors for IL-3 and IL-5. GM-CSFRα binds with low affinity to GM-CSF while the αβ heterodimeric complex facilitates a stable interaction with high affinity. Mouse GM-CSFRα is a single type I membrane protein of 387 amino acids with less than 35% homology to the human counterpart. Analysis of GM-CSFRα expression in cell populations in peripheral blood showed that all populations of granulocytes and monocytes express this receptor. Soluble form of GM-CSFRα has been described as the result of alternative splicing or metalloproteinase activity. Thus, the soluble receptor was identified in medium conditioned by human myeloid leukemia cell lines like U937, THP-1, in normal human granulocytes, and in normal human plasma. Stimulation of monocytes with GM-CSF, LPS, PMA, and the calcium ionophore A23187 rapidly up-regulates the secretion of soluble GM-CSFRα through ectodomain shedding of cell surface receptor. The soluble receptor lacks the transmembrane domain and is able to antagonize GM-CSF binding and function.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Mouse GM-CSFRα
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 60 ng/mL recombinant Mouse GM-CSF to 2 μg/mL immobilized recombinant Mouse GM-CSFRα-Fc Chimera (carrier-free). ND50 range: 0.04 – 0.24 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Distribution
-
Monocytes, granulocytes, tissue macrophages, type II alveolar epithelial cells, CD34+ progenitor cells, uterine cells, vascular endothelial cells, and fibroblasts.
- Function
- Modulate effector functions on myeloid cells, type II alveolar epithelial cells, CD34+ progenitor cells, uterine cells, vascular endothelial cells, and fibroblasts. Toll-like receptor agonists induce its expression in RAW 264.7 cells.
- Ligand/Receptor
- GM-CSF
- Biology Area
- Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors, Soluble Receptors
- Antigen References
-
- Park LS, et al. 1998. Proc Natl Acad Sci USA. 89:4295.
- Prevost GM, et al. 2002. J Immunol. 169:5679-88.
- Hamilton JA and Anderson GP. 2004. Growth Factors. 22:225-31.
- Rosas M, et al. 2007. Eur J Immunol. 37:2518-28.
- Kared H, et al. 2008. Blood. 112:2575-8.
- Bhattacharya P, et al. 2011. J Leukoc Biol. 89:235-49.
- Bhattacharya P, et al. 2015. Cytokine. 75:261-71.
- Gene ID
- 12982 View all products for this Gene ID
- UniProt
- View information about CD116 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD116 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse CD116 (GM-CSF R alpha) | W23019A | Block |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse CD116 (GM-CSF R alpha)
Recombinant mouse GM-CSF (Cat. No. 576306) (black circles) b...
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