Ultra-LEAF™ Purified anti-mouse CD182 (CXCR2) Antibody

Pricing & Availability
Clone
3F10-B3 (See other available formats)
Regulatory Status
RUO
Other Names
IL-8RB, GRO/MGSA, Cmkar2
Isotype
Mouse IgG2c, κ
Ave. Rating
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Product Citations
publications
A_3F10B3_ULEAF_CD182_Antibody_060622
C57BL/6 mouse bone marrow cells were stained with anti-mouse Ly-6G/Ly-6C APC and Ultra-LEAF™ Purified anti-mouse CXCR2 (clone 3F10-B3) (left) or purified mouse IgG2a, κ isotype control (right), followed by PE anti-mouse IgG2a.
  • A_3F10B3_ULEAF_CD182_Antibody_060622
    C57BL/6 mouse bone marrow cells were stained with anti-mouse Ly-6G/Ly-6C APC and Ultra-LEAF™ Purified anti-mouse CXCR2 (clone 3F10-B3) (left) or purified mouse IgG2a, κ isotype control (right), followed by PE anti-mouse IgG2a.
  • B_3F10B3_ULEAF_CD182_Antibody_2_060622
    Recombinant mouse CXCL2 (5 ng/mL) (Cat. No 582502) chemoattracts Baf3-mCXCR2 transfected cells (black circles) in a dose-dependent manner. Anti-mouse CXCR2 (clone 3F10-B3) inhibits the migration of Baf3-mCXCR2 transfected cells (purple circles), in a dose dependent manner. The ED50 for this effect is 1 - 4 µg/mL.
Cat # Size Price Quantity Check Availability Save
163903 100 µg £200
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163904 1 mg £509
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD182, also known as CXCR2, is a G-protein coupled receptor with 7 transmembrane regions that is involved in chemotaxis, neutrophil activation and angiogenesis. CXCR2 is expressed by neutrophils, basophils, subset of T cells, monocytes, macrophages, NK and NKT cells. Its ligands are CXCL1, CXCL2, CXCL3 and CXCL5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Pre-B cell transfected with mCXCR2
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 3F10-B3 blocks the chemotaxis towards CXCR2 ligands.

RRID
AB_2927843 (BioLegend Cat. No. 163903)
AB_2927843 (BioLegend Cat. No. 163904)

Antigen Details

Structure
G-protein coupled receptor, 7 transmembrane regions.
Distribution

Neutrophils, basophils, subset of T cells, monocytes, macrophages, and natural killer cells (NK)

Function
Chemotaxis, neutrophil activation, and angiogenesis
Ligand/Receptor
CXCL1, CXCL2, CXCL3, and CXCL5
Cell Type
Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References
  1. Spaan AN, et al. 2014. Nat Commun. 5:5438.
  2. Natsuaki Y, et al. 2014. Nat. Immunol. 11:1064-9.
  3. Hsieh CY, et al. 2014. J Immunol. 193:3693-703.
Gene ID
12765 View all products for this Gene ID
UniProt
View information about CD182 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/06/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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