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Ultra-LEAF™ Purified anti-mouse CD1d (CD1.1, Ly-38) Antibody

Pricing & Availability
Clone
1B1 (See other available formats)
Regulatory Status
RUO
Other Names
CD1, CD1.1, Ly-38
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
1B1_LEAF_020708.jpg
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ Purified 1B1, followed anti-rat IgG FITC
  • 1B1_LEAF_020708.jpg
    C57BL/6 mouse splenocytes stained with Ultra-LEAF™ Purified 1B1, followed anti-rat IgG FITC
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123531 100 µg £113
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123532 1 mg £464
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD1d, known as CD1.1 and Ly-38, is a 48 kD type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. In humans, CD1 family consists of group I proteins (CD1a, CD1b, and CD1c), group II (CD1d), and group III (CD1e). But CD1d is the only CD1 molecule has been found in mouse. Mouse CD1d has broad tissue distribution, and is found on leukocytes, dendritic cells, epithelial cells, and thymocytes. CD1d plays a role in non-peptide glycolipid antigen presentation to CD1d-restricted T cells. It has been shown that PKCδ is a critical regulator of CD1d-mediated antigen presentation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Cd1.1 cDNA-transfected RMA-S mouse T lymphoma
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IHC, IP, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistical staining, and blocking function3.
 

This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Fischer K, et al. 2004. P. Natl. Acad. Sci. USA 101:10685. (Block)
  2. Brozovic S, et al. 2004. Nat. Med. 10:535.
  3. Brossay L, et al. 1997. J. Immunol.. 159:1216. (Block)
  4. Jiang J, et al. 2012. PLoS One. 7:47487. PubMed
RRID
AB_2832442 (BioLegend Cat. No. 123531)
AB_2832442 (BioLegend Cat. No. 123532)

Antigen Details

Structure
48 kD glycoprotein, Ig superfamily
Distribution

leukocytes, dendritic cells, epithelial cells, thymocytes

Function
Antigen presentation
Ligand/Receptor
similar to MHC class I, associate with ß-microglobulin
Cell Type
Dendritic cells, Epithelial cells, Leukocytes, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Brudin N, et al. 1998. J. Immunol. 161:3271.
2. Amano M, et al. 1998. J. Immunol. 161:1710.
3. Brossay L, et al. 1997. J. Immunol. 159:1216.
4. Dougan SK, et al. 2007. Curr. Top. Microbiol. Immunol. 314:113.
5. Brutkiewicz RR, et al. 2007. Eur. J. Immunol. 37:2390.

Gene ID
12479 View all products for this Gene ID
UniProt
View information about CD1d on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/09/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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