Ultra-LEAF™ Purified anti-mouse CD254 (TRANCE, RANKL) Antibody

Pricing & Availability
Clone
W17172A (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF, Osteoprotegerin ligand, OPGL, Receptor activator of nuclear factor kappa-B ligand, RANKL, TNF-related activation-induced cytokine, TRANCE, CD254, TNFSF11
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17172A_Ultra-LEAF_CD254_Antibody_030333
Recombinant Mouse TRANCE (RANKL, TNFSF11) (carrier-free) (Cat. No. 769406) (black circles) induces osteoclast differentiation in mouse RAW.264.7 cells. Ultra-LEAF™ purified anti-mouse CD254 (TRANCE, RANKL) (clone W17172A) (purple squares) inhibits the effect of recombinant mouse TRANCE (RANKL) at 4 ng/mL in RAW.264.7 cells in a dose-dependent manner, as measured by TRAP (tartrate-resistant acid phosphatase) assay. However, the GoInVivo™ purified Rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 7 - 35 ng/mL
  • W17172A_Ultra-LEAF_CD254_Antibody_030333
    Recombinant Mouse TRANCE (RANKL, TNFSF11) (carrier-free) (Cat. No. 769406) (black circles) induces osteoclast differentiation in mouse RAW.264.7 cells. Ultra-LEAF™ purified anti-mouse CD254 (TRANCE, RANKL) (clone W17172A) (purple squares) inhibits the effect of recombinant mouse TRANCE (RANKL) at 4 ng/mL in RAW.264.7 cells in a dose-dependent manner, as measured by TRAP (tartrate-resistant acid phosphatase) assay. However, the GoInVivo™ purified Rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. ND50 range: 7 - 35 ng/mL
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602953 100 µg £245
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Description

RANKL gene encodes a type II membrane protein of 316 amino acids with a predicted molecular mass of 35 kD. RANKL is cleaved to produce a soluble form with biological activity. The shedding of membrane-bound RANKL appears to be mediated by expression of MMP14 and ADAM10. Suppression of MMP14 in primary osteoblasts increases membrane-bound RANKL and promotes osteoclastogenesis in cocultures with macrophages. Therefore, RANKL shedding seems to be an important process that down-regulates local osteoclastogenesis. Alternatively, an increased production of RANKL by osteoblastic cells leads to osteoclast differentiation, activation, and survival, which results in increased bone resorption. Binding of RANKL to its receptor RANK activates TNF receptor-associated factor 6 (TRAF6), which is linked to downstream pathways including NF-κB, c-jun N-terminal kinase (JNK) or Src. TRAF6 has been shown to be necessary for the differentiation of osteoclastic cells by enhancing Src kinase, essential for osteoclast function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse RANKL
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by the neutralization of osteoclast differentiation induced by recombinant mouse TRANCE (RANKL) (Cat. No. 769406) at 4 ng/mL in mouse RAW.264.7 cells. ND50 range: 7 - 35 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922632 (BioLegend Cat. No. 602953)

Antigen Details

Structure
Cytokine
Distribution

Predominantly expressed in monocytes, macrophages, polymorphonuclear cells, B cells. Detected in microglial cells and Treg cells. Inducible in keratinocytes and endothelial cells.

Function
Potent stimulator of osteoclast formation from precursor cells and bone-resorbing activity in mature osteoclasts. Augments the ability of dendritic cells to stimulate naïve T-cell proliferation. Activates JNK and NF-κB and induces the expression of IL-1, IL-6, IL-12, and IL-15 in dendritic cells. Crucial factor in lymphocyte development and lymph node organogenesis. Stimulates proliferation, adhesion, and IL-7 expression of thymic epithelial cells. RANKL expression, in human and murine osteoblastic cells, is stimulated by various cytokines (IL-1, TNF-α and IL-11) and calciotrophic hormones including PTH, 1, 25-dihydroxyvitamin D3 (1,25D3), and prostaglandin E2.
Interaction
T cells, dendritic cells, bone marrow derived macrophages, osteoclast precursors and mature osteoclasts
Ligand/Receptor
TNFRSF11A (Tumor necrosis factor receptor superfamily, member 11A) also called RANK and TNFRSF11B (OPG) which acts as a decoy receptor for RANKL and as a potent inhibitor of osteoclast formation.
Cell Type
Osteoclasts, T cells
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References
  1. Dehm SM, et al. 2004. Biochem Cell Biol. 82:263-74.
  2. Hikita A, et al. 2006. J Biol Chem. 281:36846-55.
  3. Kim NS, et al. 2006. Mol Cell Biol. 26:1002-13.
  4. Wada T, et al. 2006. TRENDS Mol Med. 12:17-25.
  5. Lee HW, et al. 2008. Exp Mol Med. 40:59-70.
  6. Ha J, et al. 2010. J Immunol. 184:4717-24.
Gene ID
21943 View all products for this Gene ID
UniProt
View information about CD254 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/03/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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