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Ultra-LEAF™ Purified anti-mouse CD28 (Superagonistic) Recombinant Antibody

Pricing & Availability
Clone
D665.Rec (See other available formats)
Regulatory Status
RUO
Other Names
Tp44, T44
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
D665Rec_Ultra-LEAF_-CD28_Antibody_053123
C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ Purified anti-mouse CD28 (clone D665.Rec) (left) or purified mouse IgG1, κ isotype control (right) followed by PE anti-mouse IgG1. Cells were costained with anti-mouse CD3 APC.
  • D665Rec_Ultra-LEAF_-CD28_Antibody_053123
    C57BL/6 mouse splenocytes were stained with Ultra-LEAF™ Purified anti-mouse CD28 (clone D665.Rec) (left) or purified mouse IgG1, κ isotype control (right) followed by PE anti-mouse IgG1. Cells were costained with anti-mouse CD3 APC.
  • D665Rec_Ultra-LEAF_-CD28_Antibody_2_053123
    Total T-cells from C57BL/6 mouse splenocytes were cultured with Ultra-LEAF™ purified anti-mouse CD28 (clone D665.Rec) (filled histogram) or Ultra-LEAF™ purified mouse IgG1, κ isotype control (open histogram) for 3 days. Cells were fixed and permeabilized with 70% ethanol, and then stained intracellularly with anti-mouse Ki-67 Alexa Fluor® 647. Data shown are CD4+ cells.
  • D665Rec_Ultra-LEAF_-CD28_Antibody_3_053123
    Total T-cells from C57BL/6 mouse splenocytes were labeled with CFSE, and stimulated with Ultra-LEAF™ purified anti-mouse CD28 (clone D665.Rec) (filled histogram) or Ultra-LEAF™ purified mouse IgG1, κ isotype control (open histogram) for 4 days. Data shown are CD4+ cells.
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117003 100 µg £76
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117004 1 mg £102
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD28, also known as Tp44 or T44, is a 44 kD glycoprotein. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The clone D665.Rec is able to stimulate mouse T cells to proliferate without TCR or CD3 stimulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Activ - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone D665.Rec can completely block the binding of clone D665 on target cells.

Clone D665.Rec can induce proliferation of mouse T cells without TCR or anti-CD3 stimulation.

RRID
AB_3068072 (BioLegend Cat. No. 117003)
AB_3068072 (BioLegend Cat. No. 117004)

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution

Thymocytes, peripheral T cells

Function
Costimulates T
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Costimulatory Molecules, Immuno-Oncology, Immunology
Molecular Family
CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Lenschow DJ, et al. 1996. Annu Rev Immunol. 14:233-58.
  3. Gross JA, et al. 1992. J Immunol. 149:380-8.
Gene ID
12487 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/02/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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