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Ultra-LEAF™ Purified anti-mouse CD300a Antibody

Pricing & Availability
Clone
TX41 (See other available formats)
Regulatory Status
RUO
Other Names
CD300a, MAIR-I, LMIR-I, CLM-8, CMRF-35H, IRp60
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
TX41_Purified_CD300a_Antibody_FC_061614.jpg
MAIR I-transfected cells (ST87) were stained with purified anti-mouse CD300a (clone TX41) (filled histogram) or purified rat IgG2a isotype control (open histogram), followed by anti-rat IgG PE.
  • TX41_Purified_CD300a_Antibody_FC_061614.jpg
    MAIR I-transfected cells (ST87) were stained with purified anti-mouse CD300a (clone TX41) (filled histogram) or purified rat IgG2a isotype control (open histogram), followed by anti-rat IgG PE.
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147901 100 µg £185
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Description

CD300 receptors are a group of type I transmembrane proteins, which belong to the Ig superfamily. In mice, nine members have been reported with different nomenclature systems, such as myeloid-associated Ig-like receptor (MAIR), leukocyte mono-Ig-like receptor (LMIR), DC-derived Ig-like receptor (DIgR), and CMRF-35-like molecule (CLM). The CD300 molecules act as either activating or inhibitory receptors through interactions with adaptor molecules, such as PAD12, DAP10, and FcRγ. CD300a is an inhibitory receptor that is mainly expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells. Cross-linking CD300a by an antibody can induce phosphorylation of tyrosine residues in the cytoplasmic domain, which results in recruitment of phosphatases SHIP, SHP1 and SHP2 and inhibition of cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
MAIR-I transfected
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, Activ - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation and induction of cytokine production by peritoneal macrophages.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakahashi-Oda C, et al. 2012. J. Exp. Med. 209:8. (FC, Block)
RRID
AB_2563668 (BioLegend Cat. No. 147901)

Antigen Details

Structure
Ig superfamily, three immunoreceptor tyrosine-based inhibitory motifs (ITIMs), one non-canonical ITIM
Distribution

Peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells

Function
Acts as an inhibitory receptor
Interaction
DAP12, DAP10, FcRγ
Ligand/Receptor
Phosphatidylserine
Cell Type
B cells, Dendritic cells, Eosinophils, Macrophages, Mast cells, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Yotsumoto K, et al. 2003. J. Exp. Med. 198:223.
2. Martinez-Barriocanal A, et al. 2010. J. Biol. Chem. 285:41781.
3. Borrego F. 2013. Blood. 121:1951.
4. Lankry D, et al. 2013. Eur. J. Immunol. 43:2151.

Gene ID
217303 View all products for this Gene ID
UniProt
View information about CD300a on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 10/29/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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