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Ultra-LEAF™ Purified anti-mouse CD99 Antibody

Pricing & Availability
Clone
W20091C (See other available formats)
Regulatory Status
RUO
Other Names
CD99, MIC2, HBA71, MSK5X, E2 antigen
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20091C_ULEAF_CD99_Antibody_030422
Recombinant mouse PILRα (Cat. No. 792806) (black circles) binds to immobilized recombinant mouse CD99. Ultra-LEAF™ purified anti-mouse CD99 (clone W20091C) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2b, κ isotype control (Cat. No. 400666) (gray triangles) does not have the effect. This antibody blocks the binding of 7.5 µg/mL recombinant mouse PILRα to 5 µg/mL immobilized recombinant mouse CD99. ND50 range: 0.4 - 2 µg/mL.
  • W20091C_ULEAF_CD99_Antibody_030422
    Recombinant mouse PILRα (Cat. No. 792806) (black circles) binds to immobilized recombinant mouse CD99. Ultra-LEAF™ purified anti-mouse CD99 (clone W20091C) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2b, κ isotype control (Cat. No. 400666) (gray triangles) does not have the effect. This antibody blocks the binding of 7.5 µg/mL recombinant mouse PILRα to 5 µg/mL immobilized recombinant mouse CD99. ND50 range: 0.4 - 2 µg/mL.
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604253 100 µg £283
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604254 1 mg £825
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD99 is a type I single chain transmembrane protein devoid of N-linked glycosylation sites encoded by the pseudoautosomal gene MIC2. CD99 has an apparent molecular weight of 32 kD and is widely expressed on a variety of tissues. CD99 is highly expressed on thymocytes, T cells, and T cell leukemias and lymphomas. However, it is absent on some B cell lines, fetal B cells, eosinophils, granulocytes, and the NK-cell line YT. CD99 is involved in spontaneous rosette formation with erythrocytes and may also be involved in other T-cell and hematopoietic cell adhesion pathways. CD99 has been reported to activate a caspase-independent death pathway in T cells under some conditions. CD99 interacts with a number of proteins including ferritin heavy chain 1, karyopherin beta 1, TRIP13, cyclophilin A, annexin II, and ubiquitin-conjugating enzyme E2H.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD99
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 7.5 µg/mL recombinant mouse PILRα (Cat. No. 792806) to 5 µg/mL immobilized recombinant mCD99. ND50 range: 0.4 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

RRID
AB_2922637 (BioLegend Cat. No. 604253)
AB_2922637 (BioLegend Cat. No. 604254)

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

T-cells, thymocytes, T-cell leukemias and lymphomas. It is absent on fetal B-cells, eosinophils, granulocytes, and NK cell line YT.

Function
Leukocyte migration, T-cell adhesion, caspase independent T-cell death, and spontaneous rosette formation with erythrocytes
Interaction
Annexin II, E2H, Ferritin heavy chain 1, karyopherin beta 1, and TRIP13
Ligand/Receptor
PILR-alpha and PILR-beta
Cell Type
B cells, Basophils, Dendritic cells, Fibroblasts, Macrophages, Mast cells, NK cells, Platelets, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Gelin C, et al. 1989. EMBO J. 8:3253-59. 
  2. Goodfellow PJ, et al. 1986. Science. 234:740-43. 
  3. Pettersen RD, et al. 2001. J. Immunol. 166:4931-42
Gene ID
673094 View all products for this Gene ID
UniProt
View information about CD99 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/04/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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