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Ultra-LEAF™ Purified anti-mouse IL-17A Recombinant Antibody

Pricing & Availability
Clone
QA20A33 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin 17A, IL17A, IL-17, IL17, CTLA-8 , CTLA8
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A20A33_ULEAF_IL-17A_Antibody_1_012622
Recombinant mouse IL-17A (Cat. No. 576002) (black circles) induces the production of mouse IL-6 in fetal skin mouse fibroblasts. Ultra-LEAF™ purified anti-mouse IL-17A (clone QA20A33) (purple squares) inhibits the effect of recombinant mouse IL-17A (at 5 ng/mL) in fetal skin mouse fibroblasts in a dose-dependent manner, whereas the mouse IgG1, κ isotype control (grey triangles) (Cat. No. 400165) does not have effect. The ND50 = 7 – 35 ng/mL
  • A20A33_ULEAF_IL-17A_Antibody_1_012622
    Recombinant mouse IL-17A (Cat. No. 576002) (black circles) induces the production of mouse IL-6 in fetal skin mouse fibroblasts. Ultra-LEAF™ purified anti-mouse IL-17A (clone QA20A33) (purple squares) inhibits the effect of recombinant mouse IL-17A (at 5 ng/mL) in fetal skin mouse fibroblasts in a dose-dependent manner, whereas the mouse IgG1, κ isotype control (grey triangles) (Cat. No. 400165) does not have effect. The ND50 = 7 – 35 ng/mL
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946503 100 µg £148
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946504 1 mg £486
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

IL-17A was initially identified from a subtracted cDNA library between closely related murine lymphoid cells called CTLA-8. IL-17A shares 58% homology with an open reading frame of the T-lymphotropic Herpes virus Samirii virus (viral IL-17). IL-17A belongs to a family of cytokines, which has five members: designated IL-17A-F. IL-17 is expressed by a unique lineage of CD4 T cells known as Th17 cells. This lineage selectively produces proinflammatory cytokines including IL-17, IL-17F, IL-21, and IL-22. In mice, the differentiation of this new lineage is initiated by TGFbeta-1 and IL-6 or IL-21, which activates Stat3 and induces the expression of the transcription factor retinoic acid-related orphan receptors (RORγt and RORα). IL-23, which also activates Stat3, serves to maintain Th17 cells lineage.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse IL-17A
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the induction of mouse IL-6 by recombinant mouse IL-17A (5 ng/mL) in fetal mouse skin fibroblasts. ND50 range:  7 – 35 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910507 (BioLegend Cat. No. 946503)
AB_2910507 (BioLegend Cat. No. 946504)

Antigen Details

Structure
Dimer
Distribution

Memory T lymphocytes, CD4+ T helper cells (Th17), neutrophils, CD8(+), NK, and gamma-delta T cells

Function
Potent regulator of granulopoiesis and neutrophil recruitment under normal and inflammatory conditions. Its organ overexpression increases circulating neutrophil numbers and recruitment into the organs by induction of CXCL2, IL-1β, and G-CSF. Induces CXCL1, CXCL2, CXCL5, and CXCL8 in human epithelial cells. Cooperates with TLR ligands, IL-1β, and TNF-α to enhance inflammatory reactions and stimulate production of β-defensins and other antimicrobial peptides. Th17 differentiation of human and murine naïve T cells is markedly suppressed in the presence of IFNα.
Interaction
Epithelial cells, fibroblasts, B and T lymphocytes, myelomonocytic cells, and marrow stromal cells
Ligand/Receptor
Heteromeric receptor composed of IL-17RA and IL-17RC
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Yu J, et al. 2008. Front Biosci. 13:170-7.
  2. Toy D, et al. 2006. J Immunol. 177:36-9.
  3. Benghiat FS, et al. 2009. Transplant Rev. 23:11-8.
  4. Honorati MC, et al. 2001. Rheumatology. 40:522-7.
  5. Rouvier E, et al. 1993. J Immunol. 150:5445-56.
  6. Liang SC, et al. 2007. J Immunol. 179:7791-9.
  7. Ouyang W, et al. 2008. Immunity. 28:454-67.
Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 04/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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