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- Clone
- QA19A69 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Recombinant mouse IL-3 (Cat. No. 575504) (black circles) induces the proliferation of M-NFS-60 cells. Ultra-LEAF™ purified anti-mouse IL-3 recombinant (clone QA19A69) (purple squares) inhibits the effect of recombinant mouse IL-3 (Cat. No. 575504) at 1 ng/mL of M-NFS-60 cells in a dose-dependent manner, whereas the GoInVivo™ purified mouse IgG1, κ isotype control (Cat. No. 400192) (gray triangles) does not have this effect. ND50 range: 0.05-0.15 µg/mL
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 620553 | 100 µg | £271 | ||||
| 620554 | 1 mg | £818 | ||||
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
IL-3 is the most potent growth factor for basophils followed by granulocyte-macrophage colony-stimulating factor and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and releasability. IL-3 is highly expressed by mast cells, and rapid and large amount of autocrine IL-3 production is responsible for mast cell survival by IgE in the absence of antigen. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Recombinant mouse IL-3
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by neutralizing the M-NFS-60 cell proliferation induced by recombinant mouse IL-3 (Cat. No. 575504) at 1 ng/mL. ND50 range: 0.05-0.15 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3068226 (BioLegend Cat. No. 620553)
AB_3068226 (BioLegend Cat. No. 620554)
Antigen Details
- Structure
- Cytokine
- Distribution
-
Activated T cells, Th1, Th2, mast cells, eosinophils, keratinocytes, NK cells, and endothelial cells
- Function
- Interleukine-3 is a pleiotropic factor which promotes both self-renewal and differentiation of early multipotential progenitors and is involved in inducible hematopoiesis in response to infections. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures.
- Interaction
- Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
- Ligand/Receptor
- Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Kohno M, et al. 2005. Blood.105:2059-65.
- Valent P and Dahinden CA. 2010. Curr Opin Hematol. 17:60-6.
- Kleemann R, et al. 2008. Cardiovasc Res. 79:360-76.
- Murphy JM and Young IG. 2006. Vitam Horm. 74:1-30.
- Gene ID
- 16187 View all products for this Gene ID
- UniProt
- View information about IL-3 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All IL-3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse IL-3 Recombinant Antibody | QA19A69 | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-mouse IL-3 Recombinant Antibody
Recombinant mouse IL-3 (Cat. No. 575504) (black circles) ind...
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