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Ultra-LEAF™ Purified anti-mouse IL-4 Recombinant Antibody

Pricing & Availability
Clone
QA19A66 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-4, B-cell stimulating factor-1 (BSF-1), Ia inducing factor (IaIF), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA19A66_ULEAF_IL-4_RECOM_Antibody_101421
Recombinant mouse IL-4 (Cat. No. 574306) (black circles) induces the proliferation of HT-2 mouse cells. Ultra-LEAF™ purified anti-mouse IL-4 recombinant antibody (clone QA19A66) (purple squares) inhibits the effect of recombinant mouse IL-4 in mouse HT-2 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400192) (gray triangles) does not have the effect. ND50 range: 0.1 - 0.5 µg/mL
  • QA19A66_ULEAF_IL-4_RECOM_Antibody_101421
    Recombinant mouse IL-4 (Cat. No. 574306) (black circles) induces the proliferation of HT-2 mouse cells. Ultra-LEAF™ purified anti-mouse IL-4 recombinant antibody (clone QA19A66) (purple squares) inhibits the effect of recombinant mouse IL-4 in mouse HT-2 cells in a dose-dependent manner whereas the GoInVivo™ purified mouse IgG1, κ isotype ctrl antibody (Cat. No. 400192) (gray triangles) does not have the effect. ND50 range: 0.1 - 0.5 µg/mL
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942803 100 µg £223
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942804 1 mg £715
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

IL-4 is a member of the short four-helix bundle family of cytokines. IL-4 is produced by Th2 cells in response to antigen receptor engagement. Dendritic cells can prime naïve CD4+ T cell; nevertheless, it has been described that dendritic cell mediated priming is insufficient for the development of Th2 cell-dependent immunity. Basophils have been identified as a source of IL-4 in the initial naïve CD4+ T cell priming. These cells coexpress MHC class II and IL-4 following helminth infection. Therefore, murine basophils could present antigens to naïve T cells, thus promoting Th2 cell differentiation during allergic and anti-parasitic immune responses. In addition, it was previously known that upon cross-linkage of the high affinity receptor for immunoglobulin E, IL-4 is produced by NK cells, mast cells, and basophils. Cells of hematopoietic stem cell origin express the type I receptor for IL-4 (IL-4Ra, γc), and cells of non-hematopoietic stem cell origin express type II receptor (IL-4Ra, IL-13Ra1). Type I receptor complexes are active in regulating Th2 development. In contrast, the type II receptor complex is active in regulating cells that mediate airway hypersensitivity and mucus secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Recombinant mouse IL-4
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant mouse IL-4 (Cat. No. 574306) on mouse HT-2 cells. ND50 range: 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2910503 (BioLegend Cat. No. 942803)
AB_2910503 (BioLegend Cat. No. 942804)

Antigen Details

Structure
Heterodimer, interleukin
Distribution

Th2 cells, NK T cells, mast cells, basophils, eosinophils, macrophages

Function
B-cell stimulatory cytokine, Th2 promoting, immunoglobulin isotype switching to IgG1 and IgE, upregulates MHC class II production, T-cell suppressor, regulator in humoral and adaptive immunity, decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL-12.
Interaction
T cells, B cells, mast cells, monocytes, macrophages, fibroblast, and endothelial cells
Ligand/Receptor
Type I receptor complex (IL-4Rα, γc) and type II receptor complex (IL-4Rα, IL-13Rα1)
Cell Type
Basophils, Embryonic Stem Cells, Eosinophils, Hematopoietic stem and progenitors, Macrophages, Mast cells, NKT cells
Biology Area
Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Chen. L, et al. 2004. J. Immunol. 172:2059.
  2. Maroof A, et al. 2006. Immunology 117:271.
  3. Sharma P, et al. 2008. Immunity 29:551.
  4. Heller NM, et al. 2008. Sci. Signal 1:ra17.
  5. LaPorte SL, et al. 2008. Cell 132:259.
  6. Perrigoue JG, et al. 2009. Nat. Immunol. 10:697.
  7. Schneider E, et al. 2010. Eur. Cytokine Netw. 21:142.
  8. Leavy O. 2011. Nat. Rev. Immunol. doi:10.1038/nri2908.
Gene ID
16189 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 2    Revision Date: 09/26/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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