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Ultra-LEAF™ Purified anti-mouse VEGF-A Antibody

Pricing & Availability
Clone
2G11-2A05 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial growth factor (VEGF)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
2G11-2A05_LEAF_VEGF-A_Antibody_WB_051016
Western blot analysis of 50 ng of recombinant mouse VEGF-164 (lane 1), recombinant mouse VEGF-120 (lane 2), recombinant human VEGF-165 (lane 3), recombinant human VEGF-121 (lane 4), and recombinant human VEGF-C (lane 5) using LEAF™ purified anti-mouse VEGF-A (clone 2G11-2A05) antibody.
  • 2G11-2A05_LEAF_VEGF-A_Antibody_WB_051016
    Western blot analysis of 50 ng of recombinant mouse VEGF-164 (lane 1), recombinant mouse VEGF-120 (lane 2), recombinant human VEGF-165 (lane 3), recombinant human VEGF-121 (lane 4), and recombinant human VEGF-C (lane 5) using LEAF™ purified anti-mouse VEGF-A (clone 2G11-2A05) antibody.
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512809 100 µg £185
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512810 1 mg £490
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

VEGF-A, also known as VEGF (vascular endothelial growth factor), is the founding member of the family including VEGF (VEGF-A), VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. Vascular endothelial growth factors (VEGFs) are a family of secreted polypeptides with a highly conserved receptor-binding cystine-knot structure similar to that of the platelet-derived growth factors.VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis.VEGF-A is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. Oxygen tension is a key physiological regulator of VEGF-A gene expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
N-terminal 24 aa sequence of murine VEGF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
Neut, ELISA, IHC-F, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Neutralization: The Ultra-LEAF™ Purified antibody is recommended for neutralization of mouse VEGF-A bioactivity in vivo2,4 and in vitro2.
Western Blotting: It is suggested to use 0.5 - 2 µg/ml for WB2.
Immunohistochemistry: Clone 2G11-2A05 is recommended for IHC on saponin-treated frozen tissue sections at a concentration range of 5-10 µg/ml.
ELISA or ELISPOT: The Ultra-LEAF™ Purified 2G11-2A05 antibody is useful in a mouse VEGF-A ELISA or ELISPOT assay.
Functional Assay: The Ultra-LEAF™ Purified 2G11-2A05 antibody is useful in a mouse VEGF-A functional assay2.

Application References

(PubMed link indicates BioLegend citation)
  1. Wuest TR, et al. 2009. J. Exp Med. 207;101. (Neut) PubMed
  2. Basu A, et al. 2008. Cancer Res. 68:5689. (Neut, WB, FA)
  3. Kumar V, et al. 2010. Blood 115:4725. PubMed
  4. Lu R, et al. 2012. Cancer Res. 72:2239. (Neut) PubMed
  5. Jayarman P, et al. 2012. J. Immunol. 188:5365. PubMed
  6. Kather JN, et al. 2014. Invest Ophthalmol Vis Sci. 55:4295. PubMed
Product Citations
  1. Ke Z, et al. 2024. Liver Int. 776:44. PubMed
  2. Li S, et al. 2023. Nat Commun. 7709:14. PubMed
  3. Shi L, et al. 2021. Immunity. . PubMed
RRID
AB_2814440 (BioLegend Cat. No. 512809)
AB_2814440 (BioLegend Cat. No. 512810)

Antigen Details

Structure
Cystine-knot growth factors
Distribution

Aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells

Function
Angiogenesis
Cell Targets
Endothelial cells
Receptors
VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1)
Cell Type
Macrophages
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Contreras A, et al. 2007. Am. J. Transpl. 7:155.
2. Lambrechts D and Carmeliet P. 2007. Biochim. Biophys. Acta. 1762:1109.
3. Tong JP and Yao YF. 2006. Clin. Biochem. 39:267.
4. Ferrara N, et al. 2003. Nat. Med. 9:669.
5. Shibuya M. 2001. Cell Struct. Funct. 26:25.
6. Senger DR, et al. 1983. Science. 219:983.

Regulation
HIF-1, hypoxiaresponsiveness
Gene ID
22339 View all products for this Gene ID
UniProt
View information about VEGF-A on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/28/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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