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Ultra-LEAF™ Purified anti-Syntrophin Antibody

Pricing & Availability
Clone
1351 (See other available formats)
Regulatory Status
RUO
Other Names
Dystrophin-associated protein A1 acidic component 1, Pro-TGF-alpha cytoplasmic domain-interacting protein 1, TACIP1, Syntrophin-1, SNT1, LQT12,dJ1187J4.5
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1351_PURE_Syntrophin_Antibody_WB_090915
Western blot showing human and mouse reactivity with clone 1351, which was tested on 15 µg/lane of HeLa, PC-3, 3T3, and HUVEC lysates and was tested at 1 µg/ml. The blot was incubated with HRP anti-mouse IgG secondary antibody and GAPDH antibody was used as a positive control.
  • 1351_PURE_Syntrophin_Antibody_WB_090915
    Western blot showing human and mouse reactivity with clone 1351, which was tested on 15 µg/lane of HeLa, PC-3, 3T3, and HUVEC lysates and was tested at 1 µg/ml. The blot was incubated with HRP anti-mouse IgG secondary antibody and GAPDH antibody was used as a positive control.
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845103 100 µg £245
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Description

Syntrophins are a family of five 60 kD adapter proteins that use their multiple protein interaction domains (two PHDs and a PDZ) to localize various signaling proteins (kinases, ion/water channels, or NOS) to specific intracellular locations. In neurons, syntrophins are known as the postsynaptic density protein (PSD) that serves to connect neurotransmitter receptors (such as nAChRs) to the cytoskeleton.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The epitope has been mapped to amino acids 108-120 within the a-syntrophin PDZ domain.

Application References

(PubMed link indicates BioLegend citation)
  1. Froehner S, et al. 1987. J. Cell Biol. 104:1633. (WB, IHC)
  2. Gee S, et al. 1998. J. Neurosci. 18:128. (IP)
  3. Ort T, et al. 2001. EMBO 20:4013. (IP, WB)
  4. Adams M, et al. 2001. J. Cell Biol. 155:113. (IF)
  5. Williams M, et al. 1999. J. Cell Biol. 144:1259. (IHC)
RRID
AB_2566134 (BioLegend Cat. No. 845103)

Antigen Details

Structure
Monomer and homodimer. Syntrophins consist of a conserved domain, two pleckstin homology domains, a PDZ domain, and a highly conserved C-terminal syntrophin unique (SU) domain. Expected MW: 58 kD.
Function
Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex.
Interaction
Interacts with the other members of the syntrophin family SNTB1 and SNTB2; SGCG and SGCA of the dystrophin glycoprotein complex; NOS1; GRB2; the sodium channel proteins SCN4A and SCN5A; F-actin and calmodulin; Interacts with MYOC.
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
6640 View all products for this Gene ID
UniProt
View information about Syntrophin on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/17/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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