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Alexa Fluor® 647 anti-STAT1 Phospho (Tyr701) Antibody

Pricing & Availability
Clone
A17012A (See other available formats)
Regulatory Status
RUO
Other Names
Signal Transduced Activator of Transcription 1, Transcription Factor ISGF-3 components p91/p84
Isotype
Mouse IgG1, κ
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Product Citations
publications
A17012A_A647_STAT1_Antibody_1_080519_updated.png
Human peripheral blood mononuclear cells were treated with (left) or without (right) Recombinant Human IFN-γ (carrier-free, Cat. No. 570204) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with CD4 PE and anti-STAT1 Phospho (Tyr701) (clone A17012A) Alexa Fluor® 647. Data was gated on lymphocyte and monocyte populations.
  • A17012A_A647_STAT1_Antibody_1_080519_updated.png
    Human peripheral blood mononuclear cells were treated with (left) or without (right) Recombinant Human IFN-γ (carrier-free, Cat. No. 570204) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with CD4 PE and anti-STAT1 Phospho (Tyr701) (clone A17012A) Alexa Fluor® 647. Data was gated on lymphocyte and monocyte populations.
  • A17012A_A647_STAT1_Antibody_2_062818
    Human peripheral blood monocytes were treated with (filled histogram) or without (open histogram) Recombinant Human IFN-γ (carrier free, Cat. No. 570204) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with anti-STAT1 Phospho (Tyr701) (clone A17012A) Alexa Fluor® 647.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
666409 25 tests £97
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666410 100 tests £254
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Description

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated at Tyrosine 701 (Tyr701) by receptor-associated kinases, including JAK1, JAK2, and TYK2. This results in STAT1 dimerization and subsequent translocation to the nucleus, where it functions as a transcriptional activator. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone A17012A recognizes STAT1 phosphorylated at Tyrosine 701 (Tyr701).

When using this clone for ICC, we recommend using methanol to permeabilize fixed cells.

RRID
AB_2814502 (BioLegend Cat. No. 666409)
AB_2814502 (BioLegend Cat. No. 666410)

Antigen Details

Structure
Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
Distribution

Ubiquitous tissue expression, nucleoplasmic-cytosolic distribution

Function
Immune response activation, cell signaling
Interaction
STAT1, STAT2, IRF9, ERBB4, JAK1, JAK2, TYK2, TNK1, SHP2
Biology Area
Cell Biology
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Moretti S, et al. 2017. J. Biol. Chem. 292: 1785.
  2. Wei J, et al. 2015. J. Immunol. 195: 2870.
  3. Sung PS, et al. 2015. Proc. Natl. Acad. Sci. USA. 112: 10443
  4. Ooi EL, et al. 2014. Proc. Natl. Acad. Sci. USA. 111: 1909.
  5. Wu TR, et al. 2002. J. Biol. Chem. 277: 47572.
  6. Horvath, et al. 1996. J. Virol. 70: 647.
  7. Haque SJ, et al. 1995. J. Biol. Chem. 270: 25709.
Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 Phospho Tyr701 on UniProt.org
Go To Top Version: 1    Revision Date: 06/28/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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