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Anti-HA.11 Epitope Tag Antibody (Previously Covance catalog# MMS-101R)

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# MMS-101R
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_16B12_Purified_HA.11_Antibody_WB_1_102317
Western blot analysis of cell lysates from CHO and CHO HA stable cells using HA Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (#405306). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 1_16B12_Purified_HA.11_Antibody_WB_1_102317
    Western blot analysis of cell lysates from CHO and CHO HA stable cells using HA Mouse primary antibody and HRP Goat anti-Mouse secondary antibody (#405306). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 2_16B12_HAdot11_Epitope_Tag_Antibody_IF_112817
    Immunofluorescence staining of HeLa cells transfected without (A) or with (B, C) multiple tag plasmid using anti-HA Mouse primary Antibody (Clone 16B12). 2 µg/ml (1:250 dilution) Alexa Fluor® 594 (red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/30, and (B, C) is 1/150. Anti-HA Mouse primary Antibody concentrations for (A, B) is (1/1000 dilution), (C) is (1/2000 dilution).
  • 3_16B12_Purified_HA.11_Antibody_IF_021915.jpg
    Immunofluorescence of HA.11 tagged Sbhlp protein. Photo courtesy of J Brown and I Davis, UCSF.
  • 4_16B12_Purified_HA.11_Antibody_WB_2_102317
    Immunoprecipitation of HA protein from CHO and CHO-HA stable cell extracts using HA Mouse primary antibody. Western blot analysis was performed using Direct-Blot™ HRP anti-HA (#901519). Direct-Blot™ HRP anti-β-actin (#643807) was used as a lysate loading control.
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901516 25 µL £61
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901513 200 µL £196
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901514 500 µL £313
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901515 1 mL £505
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Preparation
Ascites
Concentration
The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/mL.
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

WB - Quality tested
ICC, IP - Verified
FC, Purification - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:1000 – 1:5000 . For immunocytochemistry, the suggested use of this reagent is a dilution of 1:1000 - 1:2000 is suggested. For immunohistochemistry, the suggested use of this reagent is a dilution of 1:1,000 is suggested. For immunoprecipitation, the suggested use of this reagent is a dilution of 1:150 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
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RRID
AB_2820200 (BioLegend Cat. No. 901516)
AB_2820200 (BioLegend Cat. No. 901513)
AB_2820200 (BioLegend Cat. No. 901514)
AB_2820200 (BioLegend Cat. No. 901515)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org
Go To Top Version: 9    Revision Date: 01/26/2021

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