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APC anti-human IFN-γ Antibody

Pricing & Availability
Clone
B27 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
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Product Citations
publications
B27_APC_IFN-y_Antibody_1_102324
PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellularly stained with B27 APC and CD3 (HIT3a) PE/Cyanine5
  • B27_APC_IFN-y_Antibody_1_102324
    PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes intracellularly stained with B27 APC and CD3 (HIT3a) PE/Cyanine5
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506510 100 tests £228
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, African Green, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli-expressed recombinant human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Flow Cytometry2: The fluorochrome-labeled B27 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-? -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,3,6,7: The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 506531).

Application References

(PubMed link indicates BioLegend citation)
  1. Favre C, et al. 1989. Molec. Immunol. 26:17. (Neut)
  2. Kaur A, et al. 2002. J Virol. 76:3646.
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
  4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  5. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  6. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 9:942-9. (Neut)
  7. Gangur V, et al. 1998. FASEB J. 12:705-13. (Neut)
Product Citations
  1. Huseni MA, et al. 2023. Cell Rep Med. 4:100878. PubMed
  2. Han Z, et al. 2022. J Invest Dermatol. 142:603. PubMed
  3. Yen M, et al. 2022. Cell. 185:1414. PubMed
  4. Yang Y, et al. 2023. MBio. 14:e0328522. PubMed
  5. Saeland E, et al. 2023. NPJ Vaccines. 8:45. PubMed
  6. Nesterenko PA, et al. 2021. Cell Rep. 37:110167. PubMed
  7. Laurent A, et al. 2014. PLoS One. 9:103683. PubMed
  8. Seo YB, et al. 2021. Vaccines (Basel). 9: . PubMed
  9. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  10. Cheng L, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  11. Costa-Martins AG, et al. 2021. Cell Rep Med. 2:100465. PubMed
  12. Shi Z, et al. 2021. Ann Clin Transl Neurol. 8:43. PubMed
  13. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  14. Blandine Monel et al. 2019. Cell reports. 27(1):142-153 . PubMed
  15. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  16. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
  17. Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed
  18. Alrubayyi A, et al. 2021. Nat Commun. 12:5839. PubMed
  19. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  20. Godbersen C, et al. 2017. Mol Cancer Ther. 16:1335. PubMed
  21. Zhuang X, et al. 2019. Cancer Immunol Res. 0.94375. PubMed
  22. Johnson JK, et al. 2022. Methods Mol Biol. 2470:641. PubMed
  23. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  24. Matsuzawa T, et al. 2017. J Dermatol Sci. 10.1016/j.jdermsci.2017.03.015. PubMed
  25. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  26. Qiu X, et al. 2013. Sci Transl Med. 16:207. PubMed
  27. Robert R, et al. 2017. PLoS One. 10.1371/journal.pone.0184278. PubMed
  28. Min HK, et al. 2021. Immune Netw. 21:e43. PubMed
  29. Marzan-Rivera N, et al. 2022. iScience. 25:104764. PubMed
  30. Lee IK, et al. 2020. Cancers (Basel). 12:. PubMed
  31. Nesterenko PA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
RRID
AB_315443 (BioLegend Cat. No. 506510)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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