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APC anti-mouse CD135 Antibody

Pricing & Availability
Clone
A2F10 (See other available formats)
Regulatory Status
RUO
Other Names
Flk-2, Flt3, Ly-72
Isotype
Rat IgG2a, κ
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Product Citations
publications
A_A2F10_APC_121509
C57BL/6 bone marrow cells stained with A2F10 APC and CD45R/B220 (RA3-6B2) plus CD11b (M1/70) PE
  • A_A2F10_APC_121509
    C57BL/6 bone marrow cells stained with A2F10 APC and CD45R/B220 (RA3-6B2) plus CD11b (M1/70) PE
  • B_A2F10_121509
    C57BL/6 bone marrow cells stained with rat IgG2a APC isotype control and and CD45R/B220 (RA3-6B2) plus CD11b (M1/70) PE
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135309 25 µg £65
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135310 100 µg £183
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Description

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Flt3 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Sergejeva S, et al. 2004. Blood 103:1270.
  2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
  3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations
  1. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  2. Han X, et al. 2023. J Clin Invest. 133:. PubMed
  3. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  4. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  5. Song R, et al. 2022. Immunology. 165:274. PubMed
  6. Tu J, et al. 2023. Ann Rheum Dis. 82:198. PubMed
  7. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  8. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  9. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  10. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  11. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  12. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  13. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  14. Schlager S, et al. 2015. J Leukoc Biol. 98: 837 - 850. PubMed
  15. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  16. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  17. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  18. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  19. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  20. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  21. Gao S, et al. 2022. Cell Res. 32:38. PubMed
  22. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  23. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
  24. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  25. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  26. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
  27. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  28. Oppezzo A, et al. 2020. J Clin Invest. 130:1377. PubMed
  29. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  30. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  31. Li Z, et al. 2017. Sci Rep. 7:40380. PubMed
  32. Giridhar P, et al. 2016. J Immunol. 197(11):4228-4239. PubMed
  33. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
RRID
AB_1953264 (BioLegend Cat. No. 135309)
AB_1953264 (BioLegend Cat. No. 135310)

Antigen Details

Structure
A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor
Flk-2/FLT3 ligand
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID
14255 View all products for this Gene ID
UniProt
View information about CD135 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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