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APC anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Regulatory Status
RUO
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
281-2_APC_1_120911
C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown is from gated on lymphoid cell population.
  • 281-2_APC_1_120911
    C57BL/6 mouse bone marrow cells were stained with CD19 FITC and CD138 (clone 281-2) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown is from gated on lymphoid cell population.
  • 281-2_APC_2_120911
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
142505 25 µg £107
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142506 100 µg £258
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Mi Z, et al. 2021. Vaccines (Basel). 10: . PubMed
  2. Su C, et al. 2022. NPJ Vaccines. 7:168. PubMed
  3. Kumpunya S, et al. 2023. Front Immunol. 13:1010764. PubMed
  4. Xiong E, et al. 2023. Front Immunol. 13:1083119. PubMed
  5. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  6. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  7. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  8. Yao W, et al. 2019. Nature. 568:410. PubMed
  9. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
  10. Delgado-Benito V, et al. 2020. J Exp Med. 217:00:00. PubMed
  11. Xiong Y, et al. 2018. Int J Clin Exp Pathol. 3.803472222. PubMed
  12. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  13. Wang Z, et al. 2021. J Virol. 95:e0141421. PubMed
  14. Utley A, et al. 2020. Cell Rep. 31:107815. PubMed
  15. Chen F et al. 2018. Cell reports. 25(10):2775-2783 . PubMed
  16. Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed
  17. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  18. Li Z, et al. 2021. Cell Metabolism. 33(1):78-93.e7. PubMed
  19. Zhang YN, et al. 2020. EBioMedicine. 56:102819. PubMed
  20. Roy K, et al. 2019. Immunity. 50:616. PubMed
  21. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  22. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  23. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  24. Castiblanco D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01833. PubMed
  25. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  26. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  27. Chen C, et al. 2021. J Virol. 95:e0082921. PubMed
  28. Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed
  29. Han S, et al. 2016. Arthritis Res Ther. 0.975. PubMed
  30. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  31. Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
  32. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  33. Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed
  34. Price MJ, et al. 2019. J Immunol. 203:2121. PubMed
  35. Xiong Y, et al. 2019. BMC Immunol. 20:24. PubMed
  36. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  37. Zhang Y, et al. 2022. Front Pharmacol. 13:952775. PubMed
  38. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  39. Lightman SM, et al. 2020. Cell Reports. 1.086805556. PubMed
  40. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  41. Alrefai H, et al. 2016. Nat Commun. 7:11724. PubMed
  42. Whiteley AM et al. 2017. eLife. 6 pii: e26435. PubMed
RRID
AB_10960141 (BioLegend Cat. No. 142505)
AB_10960141 (BioLegend Cat. No. 142506)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD138 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD138 (Syndecan-1) 281-2 FC,ICC,IHC-P,IHC-F
PE anti-mouse CD138 (Syndecan-1) 281-2 FC
APC anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1) 281-2 FC
PerCP/Cyanine5.5 anti-mouse CD138 (Syndecan-1) 281-2 FC
Biotin anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Cyanine7 anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 605™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 650™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 711™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Brilliant Violet 510™ anti-mouse CD138 (Syndecan-1) 281-2 FC
Alexa Fluor® 647 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Dazzle™ 594 anti-mouse CD138 (Syndecan-1) 281-2 FC
APC/Cyanine7 anti-mouse CD138 (Syndecan-1) 281-2 FC
TotalSeq™-A0810 anti-mouse CD138 (Syndecan-1) 281-2 PG
Brilliant Violet 785™ anti-mouse CD138 (Syndecan-1) 281-2 FC
TotalSeq™-C0810 anti-mouse CD138 (Syndecan-1) 281-2 PG
TotalSeq™-B0810 anti-mouse CD138 (Syndecan-1) 281-2 PG
PE/Cyanine5 anti-mouse CD138 (Syndecan-1) 281-2 FC
APC/Fire™ 750 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Fire™ 640 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Fire™ 810 anti-mouse CD138 (Syndecan-1) 281-2 FC
PE/Fire™ 700 anti-mouse CD138 (Syndecan-1) 281-2 FC
Spark Red™ 718 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™) 281-2 FC
Spark Blue™ 574 anti-mouse CD138 (Syndecan-1) (Flexi-Fluor™) 281-2 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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