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APC anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
C9B7W_APC_090310
Con A-stimulated (day-3) C57BL/6 splenocytes stained with C9B7W APC
  • C9B7W_APC_090310
    Con A-stimulated (day-3) C57BL/6 splenocytes stained with C9B7W APC
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
125209 25 µg £65
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125210 100 µg £170
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Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations
  1. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  2. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  3. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  4. Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed
  5. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  6. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  7. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  8. DeLong JH, et al. 2019. Immunohorizons. 3:13. PubMed
  9. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  10. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  11. Sande O, et al. 2016. J Immunol. 196: 691 - 702. PubMed
  12. Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed
  13. Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed
  14. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  15. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  16. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  17. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  18. Miyakoda M, et al. 2012. J Immunol. 189:4396. PubMed
  19. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  20. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
  21. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  22. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  23. Li M, et al. 2020. J Immunother Cancer. 8:00. PubMed
RRID
AB_10639935 (BioLegend Cat. No. 125209)
AB_10639935 (BioLegend Cat. No. 125210)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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