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APC/Cyanine7 anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
6D5_APCCyanine7_CD19_Antibody_031819_updated.png
C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
  • 6D5_APCCyanine7_CD19_Antibody_031819_updated.png
    C57BL/6 splenocytes were stained with CD3 FITC and CD19 (clone 6D5) APC/Cyaninie7 (left) or Rat IgG2a, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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115529 25 µg £77
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115530 100 µg £157
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Laidlaw BJ, et al. 2020. Nat Immunol. 1.626388889. PubMed
  2. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  3. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  4. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  5. Pani F, et al. 2021. Endocrinology. 162:. PubMed
  6. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  7. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  8. Schnell A, et al. 2021. Cell. 184:6281. PubMed
  9. Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed
  10. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  11. Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed
  12. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  13. Pérez-Torrado V, et al. 2022. Bio Protoc. 12:. PubMed
  14. Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed
  15. Zhang Z, et al. 2023. Aging Dis. 14:966. PubMed
  16. Lütge M, et al. 2023. Nat Immunol. . PubMed
  17. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  18. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  19. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  20. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  21. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
  22. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
  23. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  24. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  25. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  26. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  27. Tang J, et al. 2018. Mol Cell Biol. 38:e00103. PubMed
  28. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
  29. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  30. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  31. Chikina AS, et al. 2020. Cell. 183:411. PubMed
  32. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  33. Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed
  34. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  35. Trott DW, et al. 2021. Geroscience. 43:1331. PubMed
  36. Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed
  37. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  38. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  39. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  40. Bresque M, et al. 2022. J Biol Chem. :101711. PubMed
  41. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
  42. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  43. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  44. Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed
  45. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  46. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  47. Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed
  48. Wei SC, et al. 2020. Cancer Discov. . PubMed
  49. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  50. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  51. Kim P, et al. 2015. J Exp Med. 212:633. PubMed
  52. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  53. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  54. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  55. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  56. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  57. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  58. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  59. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  60. Xu J et al. 2018. Cell. 173(3):762-775 . PubMed
  61. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  62. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  63. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  64. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  65. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  66. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  67. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  68. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  69. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  70. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  71. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  72. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  73. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  74. Hassan AO, et al. 2020. Cell. 183:169. PubMed
  75. Carbone C, et al. 2021. J Immunother Cancer. 9:. PubMed
  76. Elkhatib SK, et al. 2021. Biol Psychiatry Glob Open Sci. 1:190. PubMed
  77. Colombo M, et al. 2022. iScience. 25:105042. PubMed
  78. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  79. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  80. Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed
  81. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  82. Fischer FA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  83. Trott DW, et al. 2021. J Physiol. 599:3973. PubMed
  84. Engblom C, et al. 2017. Science. 358:6367. PubMed
  85. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  86. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  87. Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed
  88. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  89. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  90. Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed
  91. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
  92. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  93. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  94. Du P, et al. 2022. Front Pharmacol. 13:805508. PubMed
  95. Strawbridge R, et al. 2016. Diabetes. 65: 2888 - 2899. PubMed
  96. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  97. La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed
  98. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  99. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  100. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  101. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  102. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  103. Chavez JS, et al. 2022. Cells. 11:. PubMed
  104. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  105. Wilson CS, et al. 2021. JCI Insight. 6:. PubMed
  106. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  107. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
  108. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  109. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  110. Cheng Q, et al. 2020. Eur J Immunol. 50:284. PubMed
RRID
AB_830706 (BioLegend Cat. No. 115529)
AB_830706 (BioLegend Cat. No. 115530)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD19 6D5 FC
Biotin anti-mouse CD19 6D5 FC
FITC anti-mouse CD19 6D5 FC
PE anti-mouse CD19 6D5 FC
PE/Cyanine5 anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Cyanine7 anti-mouse CD19 6D5 FC
Alexa Fluor® 488 anti-mouse CD19 6D5 FC
Alexa Fluor® 647 anti-mouse CD19 6D5 FC,IHC-F
Pacific Blue™ anti-mouse CD19 6D5 FC
Alexa Fluor® 700 anti-mouse CD19 6D5 FC
APC/Cyanine7 anti-mouse CD19 6D5 FC
PerCP anti-mouse CD19 6D5 FC
PerCP/Cyanine5.5 anti-mouse CD19 6D5 FC
Alexa Fluor® 594 anti-mouse CD19 6D5 IHC-F,FC
Brilliant Violet 421™ anti-mouse CD19 6D5 FC,IHC-F
Brilliant Violet 570™ anti-mouse CD19 6D5 FC
Brilliant Violet 605™ anti-mouse CD19 6D5 FC
Brilliant Violet 650™ anti-mouse CD19 6D5 FC
Brilliant Violet 785™ anti-mouse CD19 6D5 FC
Brilliant Violet 510™ anti-mouse CD19 6D5 FC
Purified anti-mouse CD19 (Maxpar® Ready) 6D5 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD19 6D5 FC
Brilliant Violet 711™ anti-mouse CD19 6D5 FC
APC/Fire™ 750 anti-mouse CD19 6D5 FC
TotalSeq™-A0093 anti-mouse CD19 6D5 PG
Brilliant Violet 750™ anti-mouse CD19 6D5 FC
TotalSeq™-B0093 anti-mouse CD19 6D5 PG
Spark Blue™ 550 anti-mouse CD19 6D5 FC
Spark NIR™ 685 anti-mouse CD19 6D5 FC
TotalSeq™-C0093 anti-mouse CD19 6D5 PG
Ultra-LEAF™ Purified anti-mouse CD19 6D5 FC,CyTOF®,IHC-F
PE/Fire™ 640 anti-mouse CD19 Antibody 6D5 FC
Spark YG™ 581 anti-mouse CD19 6D5 FC
APC/Fire™ 810 anti-mouse CD19 6D5 FC
Spark YG™ 570 anti-mouse CD19 6D5 IHC-F
Spark Blue™ 574 anti-mouse CD19 Antibody 6D5 FC
Spark Blue™ 515 anti-mouse CD19 6D5 FC
Spark UV™ 387 anti-mouse CD19 6D5 FC
Spark Red™ 718 anti-mouse CD19 (Flexi-Fluor™) 6D5 FC
Go To Top Version: 3    Revision Date: 03/18/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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