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Biotin anti-mouse IgM Antibody

Pricing & Availability
Clone
RMM-1 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Rat IgG2a, κ
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Product Citations
publications
RMM-1_Biotin_090707
C57BL/6 Mouse splenocytes stained with biotinylated RMM-1, followed by Sav-PE
  • RMM-1_Biotin_090707
    C57BL/6 Mouse splenocytes stained with biotinylated RMM-1, followed by Sav-PE
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406503 50 µg £57
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406504 500 µg £137
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
Product Citations
  1. Lai M, et al. 2016. Nat Commun. 7:12207. PubMed
  2. Gonzalez-Martin A, et al. 2016. Nat Immunol. 17:433-440. PubMed
  3. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  4. Suzuki M, et al. 2021. Sci Adv. 7:. PubMed
  5. Dong S, et al. 2021. Nature. 591:117. PubMed
  6. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  7. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
  8. Boulianne B, et al. 2013. J Immunol. 191:4521. PubMed
  9. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  10. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  11. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  12. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  13. Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed
  14. Yamada H, et al. 2018. J Immunol. 201:3492. PubMed
RRID
AB_315053 (BioLegend Cat. No. 406503)
AB_315053 (BioLegend Cat. No. 406504)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
16019 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 01/29/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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