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Brilliant Violet 421™ anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
UCHT1_BV421_032911
Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • UCHT1_BV421_032911
    Human peripheral blood lymphocytes were stained with CD3 (clone UCHT1) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • UCHT1_BV421_CD3_Antibody_ICC_012121
    Human peripheral blood mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/mL CD3 (clone UCHT1) Brilliant Violet 421™ (blue) and 5 µg/mL CD14 (clone HCD14) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.
  • UCHT1_BV421_CD3_Antibody_IHC-F_012121
    Human frozen tonsil tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 5 µg/mL of Brilliant Violet 421™ anti-human CD3 antibody (blue) and Alexa Fluor® 647 anti-human CD19 antibody (red) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
300433 25 tests £130
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300434 100 tests £261
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Additional Product Notes

View more applications data using this product in flow cytometry and to study phosphorylation kinetics in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kim S, et al. 2023. Front Immunol. 14:1062365. PubMed
  2. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
  3. Kfoury Y, et al. 2021. Cancer Cell. 39:1464. PubMed
  4. Beaudoin-Bussières G, et al. 2022. Viruses. 14: . PubMed
  5. Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed
  6. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  7. Cheng C, et al. 2022. Cancers (Basel). 14: . PubMed
  8. Lee Y, et al. 2020. J Innate Immun. 1:. PubMed
  9. Yoshitomi H, et al. 2018. Nat Commun. 2.9875. PubMed
  10. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  11. Girard MC, et al. 2021. Sci Rep. 11:5511. PubMed
  12. Mimitou EP, et al. 2021. Nat Biotechnol. 39:1246. PubMed
  13. Svensson A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183268. PubMed
  14. Ott PA, et al. 2017. Nature. 547:217. PubMed
  15. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  16. Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed
  17. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  18. Such L, et al. 2020. J Clin Invest. 4266:130. PubMed
  19. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  20. Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
  21. Saluzzo S, et al. 2022. STAR Protoc. 3:101470. PubMed
  22. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  23. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  24. Georg P, et al. 2022. Cell. 185:493. PubMed
  25. Lundell A, et al. 2017. Sci Rep. 7:39904. PubMed
  26. Merkt W, et al. 2021. BMC Immunol. 22:26. PubMed
  27. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  28. Franchini DM et al. 2019. Cell reports. 26(1):94-107 . PubMed
  29. Lanz AL, et al. 2021. Cell Reports. 36(2):109375. PubMed
  30. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
  31. Baguma R, et al. 2017. PLoS One. 10.1371/journal.pone.0184563. PubMed
RRID
AB_10962690 (BioLegend Cat. No. 300433)
AB_10962690 (BioLegend Cat. No. 300434)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
Go To Top Version: 3    Revision Date: 01/21/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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