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Brilliant Violet 421™ anti-mouse CD135 Antibody

Pricing & Availability
Clone
A2F10 (See other available formats)
Regulatory Status
RUO
Other Names
Flk-2, Flt3, Ly-72
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A2F10_BV421_CD135_Antibody_FC_1_072313.jpg
C57 BL/6 bone marrow cells stained with CD45R/B220 APC+ CD11b APC and CD135 (clone A2F10) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • A2F10_BV421_CD135_Antibody_FC_1_072313.jpg
    C57 BL/6 bone marrow cells stained with CD45R/B220 APC+ CD11b APC and CD135 (clone A2F10) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • A2F10_BV421_CD135_Antibody_FC_2_072313.jpg
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
135313 125 µL £125
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135315 50 µg £174
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135314 500 µL £275
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Description

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Flt3 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of the this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Sergejeva S, et al. 2004. Blood 103:1270.
  2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
  3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations
  1. Zhou L, et al. 2022. PLoS Negl Trop Dis. 16:e0010851. PubMed
  2. Liu X, et al. 2022. Nutrients. 14: . PubMed
  3. Safi F 2023. STAR Protocols. 4(1):101965. PubMed
  4. Chen Y, et al. 2023. Stem Cells Int. 2023:7482546. PubMed
  5. Li S, et al. 2022. Elife. 11:. PubMed
  6. Zhou W, et al. 2019. Cell Syst. 0.597916667. PubMed
  7. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  8. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  9. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  10. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  11. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  12. Bennion BG, et al. 2020. Cell Reports. 31(11):107771. PubMed
  13. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  14. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  15. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  16. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  17. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  18. Oka Y, et al. 2020. Sci Adv. 6:. PubMed
  19. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  20. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  21. Young K, et al. 2021. Cell Stem Cell. . PubMed
  22. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
RRID
AB_2562338 (BioLegend Cat. No. 135313)
AB_2562338 (BioLegend Cat. No. 135315)
AB_2562338 (BioLegend Cat. No. 135314)

Antigen Details

Structure
A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor
Flk-2/FLT3 ligand
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID
14255 View all products for this Gene ID
UniProt
View information about CD135 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 02/25/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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