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Brilliant Violet 650™ anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
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Product Citations
publications
RM4-5_BV650_020612
C57BL/6 mouse splenocytes were stained with CD3 PE and CD4 (clone RM4-5) Brilliant Violet 650™.
  • RM4-5_BV650_020612
    C57BL/6 mouse splenocytes were stained with CD3 PE and CD4 (clone RM4-5) Brilliant Violet 650™.
Compare all formats See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability Save
100545 125 µL £143
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100555 50 µg £187
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100546 500 µL £298
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  2. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  3. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
  4. Pierson M, et al. 2021. Curr Protoc. 1:e53. PubMed
  5. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  6. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  7. Kuehm LM, et al. 2021. Cancer Immunol Res. 9:227. PubMed
  8. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  9. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  10. Liu J, et al. 2023. Nat Commun. 14:2806. PubMed
  11. Wilden A, et al. 2022. Front Immunol. 13:991295. PubMed
  12. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  13. Nato G, et al. 2021. Sci Rep. 11:651. PubMed
  14. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  15. Larsen SE, et al. 2021. Sci Rep. 11:9040. PubMed
  16. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  17. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  18. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  19. Marfil-Garza BA, et al. 2022. Am J Transplant. 22:1101. PubMed
  20. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  21. Du X, et al. 2018. Proc Natl Acad Sci U S A. 115:E11731. PubMed
  22. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  23. Li J, et al. 2020. Cancer Discov. . PubMed
  24. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  25. Klessing S, et al. 2020. Vaccines (Basel). 8:. PubMed
  26. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  27. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  28. Hossain DMS, et al. 2018. J Clin Invest. 128:644. PubMed
  29. Li J, et al. 2018. Immunity. 49:178. PubMed
  30. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  31. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
  32. Casulli J, et al. 2019. Nat Commun. 10:2121. PubMed
  33. Horkova V, et al. 2020. Cell Reports. 30(5):1504-1514.e7.. PubMed
  34. Chen YG, et al. 2020. Molecular Cell. 76(1):96-109. PubMed
  35. Ullrich L, et al. 2021. Front Immunol. 12:729607. PubMed
  36. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
  37. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  38. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  39. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  40. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  41. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  42. Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed
  43. Georgiadou A, et al. 2022. Elife. 11:. PubMed
  44. Tannig P, et al. 2020. Vaccines (Basel). 8:. PubMed
  45. Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed
  46. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  47. Scharschmidt TC, et al. 2017. Cell Host Microbe. 1.199305556. PubMed
  48. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  49. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  50. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  51. Schulze J, et al. 2021. Stroke. 52:2939. PubMed
  52. Mathur AN, et al. 2019. Immunity. 50:655. PubMed
  53. Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed
  54. Hester AK, et al. 2022. Cell Rep. 38:110363. PubMed
  55. Louise V Webb et al. 2019. Immunity. 50(2):348-361 . PubMed
RRID
AB_11126142 (BioLegend Cat. No. 100545)
AB_11126142 (BioLegend Cat. No. 100555)
AB_11126142 (BioLegend Cat. No. 100546)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 2    Revision Date: 01/29/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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