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Direct-Blot™ HRP anti-β-actin Antibody

Pricing & Availability
Clone
W16197A (See other available formats)
Regulatory Status
RUO
Other Names
ACTB, Actin, cytoplasmic 1, Beta-actin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16197A_DBHRP_beta-actin_Antibody_032918
Whole cell lysates (15 µg protein) from HeLa was resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:10,000 diluted (0.01 µg/mL), 1:40,000 diluted (0.0025 µg/mL),1:100,000 diluted (0.001 µg/mL) Direct-Blot™ HRP anti-β-actin Antibody, clone W16197A, and 1:10,000 diluted (0.01 µg/mL) purified anti-β-actin Antibody, clone W16197A (Cat. No. 664801) (upper). Proteins were visualized using an 1:3000 diluted HRP anti rat-IgG secondary antibody (Cat. No.405405) for purified anti-β-actin Antibody and chemiluminescence detection. Ponceau S staining was used as loading control (lower). Lane M: MW ladder.
  • W16197A_DBHRP_beta-actin_Antibody_032918
    Whole cell lysates (15 µg protein) from HeLa was resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:10,000 diluted (0.01 µg/mL), 1:40,000 diluted (0.0025 µg/mL),1:100,000 diluted (0.001 µg/mL) Direct-Blot™ HRP anti-β-actin Antibody, clone W16197A, and 1:10,000 diluted (0.01 µg/mL) purified anti-β-actin Antibody, clone W16197A (Cat. No. 664801) (upper). Proteins were visualized using an 1:3000 diluted HRP anti rat-IgG secondary antibody (Cat. No.405405) for purified anti-β-actin Antibody and chemiluminescence detection. Ponceau S staining was used as loading control (lower). Lane M: MW ladder.
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664803 25 µL £70
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664804 100 µL £233
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Zebrafish, Drosophila
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human ACTB peptide (1-13 a.a.).
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.01 µg per ml (1:10,000 - 1:100,000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

Product Citations
  1. Altemus J, et al. 2022. J Cell Physiol. 237:3001. PubMed
  2. Li Y, et al. 2020. Stem Cell Res Ther. 0.815972222. PubMed
  3. Sripathi SR, et al. 2021. Mol Cell Proteomics. 20:100131. PubMed
  4. Wijeratne TU, et al. 2022. J Biol Chem. :102319. PubMed
RRID
AB_2728496 (BioLegend Cat. No. 664803)
AB_2728496 (BioLegend Cat. No. 664804)

Antigen Details

Structure
375 amino acids with a predicted molecular weight of 42 kD.
Distribution

Cytoplasm

Function
Actins are highly conserved proteins that are involved in cell motility.
Interaction
Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Gene ID
60 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org
Go To Top Version: 1    Revision Date: 03/29/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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