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HRP Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Horseradish Peroxidase, SAv-HRP
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Product Citations
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405210 1 mL £89
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA, ELISPOT, IHC or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For ELISPOT or Western blotting applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Buffered solution containing bovine protein and preservatives (methylisothiazolone, bromonitrodioxane, and other active isothiazolones).
Preparation
Streptavidin is conjugated with horseradish peroxidase under optimal conditions.
Storage & Handling
The streptavidin-HRP solution should be stored undiluted between 2°C and 8°C. Avoid exposure to sodium azide.

To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

ELISA - Quality tested
ELISPOT, WB, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this Streptavidin-HRP is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:3000 to determine optimal conditions. For ELISPOT analysis, the reagent should be titrated between 1:500 - 1:1500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Streptavidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Sav-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.

Application References

(PubMed link indicates BioLegend citation)
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Go To Top Version: 6    Revision Date: 12/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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