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PE anti-human CD163 Antibody

Pricing & Availability
Clone
GHI/61 (See other available formats)
Regulatory Status
RUO
Workshop
VI M38
Other Names
GHI/61, M130, RM3/1, p155, Hemoglobin/Haptoglobin Complex Receptor, macrophage-associated antigen, ED2(rat)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
GHIslash61_PE_071108.jpg
Human peripheral blood monocytes stained with GHI/61 PE
  • GHIslash61_PE_071108.jpg
    Human peripheral blood monocytes stained with GHI/61 PE
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333605 25 tests £68
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333606 100 tests £173
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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation, western blot1, and spatial biology (IBEX)6,7.

Application References

(PubMed link indicates BioLegend citation)
  1. Pulford K, et al. 1992. Immunology 75:588. (ICC, IP, WB)
  2. Law SK, et al. 1993. Eur. J. Immunol. 23:2320.
  3. Madsen M, et al. 2004. J. Biol. Chem. 279:51561.
  4. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  5. Buttari B, et al. 2011. Atherosclerosis. 215:316. PubMed
  6. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  7. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Pan L, et al. 2022. Mol Immunol. 147:101. PubMed
  2. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  3. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  4. Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed
  5. Wang D, et al. 2022. Cells. 11:. PubMed
  6. Lafôrets F, et al. 2023. iScience. 26:106514. PubMed
  7. Nshimiyimana R, et al. 2023. Am J Hematol. 98:1000. PubMed
  8. Xu J, et al. 2023. J Immunother Cancer. 11:. PubMed
  9. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  10. Lathrop S, et al. 2015. Infect Immun . 83: 2661 - 2671. PubMed
  11. Hashimoto M, et al. 2015. J Immunol. 95: 4341 - 4350. PubMed
  12. Wu T, et al. 2022. Cell Death Dis. 13:624. PubMed
  13. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  14. He C, et al. 2021. Oncoimmunology. 1897295:10. PubMed
  15. Ma L, et al. 2021. Clinical Cancer Research. 27(6):1778-1791. PubMed
  16. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  17. Iwabuchi R, et al. 2021. Frontiers in Immunology. 12:643040. PubMed
  18. Karagianni AE, et al. 2021. Sci Rep. 11:14292. PubMed
  19. Souriant S, et al. 2019. Cell Rep. 26:3586. PubMed
  20. Huang Y, et al. 2022. Front Immunol. 13:993788. PubMed
  21. Zangeneh Z, et al. 2022. Iran J Basic Med Sci. 25:474. PubMed
  22. Mendoza-Cabrera MI, et al. 2019. Innate Immun. :1753425919864658. PubMed
  23. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  24. Wendisch D, et al. 2021. Cell. 184:6243. PubMed
  25. Genoula M, et al. 2018. Front Immunol. 9:459. PubMed
  26. Burwitz B, et al. 2014. J Leukoc Biol. 96:491. PubMed
  27. Hashimoto M, et al. 2014. Cell Death Dis. 5:1267. PubMed
  28. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  29. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  30. Liu Y, et al. 2022. Clin Transl Med. 12:e654. PubMed
  31. Nakajima S, et al. 2021. Mol Cancer Res. 19:1085. PubMed
  32. Wu Q, et al. 2022. Cancer Immunol Res. 10:182. PubMed
  33. Stempin CC, et al. 2021. Cancers (Basel). 13:. PubMed
  34. Ball M, et al. 2016. PLoS One. 11: 0149600. PubMed
  35. Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed
  36. Osman A, et al. 2014. J Immunol. 192:5083. PubMed
  37. Martins AJ, et al. 2017. Cell Systems. 4(4):379-392.e12. PubMed
  38. Wang R, et al. 2019. EBioMedicine. 40:118. PubMed
RRID
AB_1134002 (BioLegend Cat. No. 333605)
AB_1134002 (BioLegend Cat. No. 333606)

Antigen Details

Structure
134 kD (non-reduced)/155 kD (reduced) glycoprotein, scavenger receptor superfamily
Distribution

Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells

Function
Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand/Receptor
Haptoglobin-hemoglobin complex, TWEAK
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Roth J, et al. 1994 Transolantation. 57:127
2. Van den Heuvel MM, et al.1999 J. Leukoc. Biol. 66:858
3. Sulahian TH, et al. 2000 Cytokines 12:1312
4. Fabriek BO, et al. 2007 J. Neuroimmunol. 187:179

Gene ID
9332 View all products for this Gene ID
UniProt
View information about CD163 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 08/19/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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