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PE anti-human IFN-γ Antibody

Pricing & Availability
Clone
B27 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
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Product Citations
publications
B27_PE_IFN-y_Antibody_1_102324
PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 PE/Cyanine5 and then intracellular stained with B27 PE
  • B27_PE_IFN-y_Antibody_1_102324
    PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were surface stained with CD3 PE/Cyanine5 and then intracellular stained with B27 PE
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506506 25 tests £71
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506507 100 tests £169
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, African Green, Pigtailed Macaque, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli-expressed recombinant human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Flow Cytometry2: The fluorochrome-labeled B27 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-? -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,3,6,7: The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-? bioactivity (Cat. No. 506531).

Application References

(PubMed link indicates BioLegend citation)
  1. Favre C, et al. 1989. Molec. Immunol. 26:17. (Neut)
  2. Kaur A, et al. 2002. J Virol. 76:3646.
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
  4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  5. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  6. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 9:942-9. (Neut)
  7. Gangur V, et al. 1998. FASEB J. 12:705-13. (Neut)
Product Citations
  1. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  2. Mo Y, et al. 2022. Front Immunol. 12:799896. PubMed
  3. Vorkas CK, et al. 2022. J Immunol. 208:1042. PubMed
  4. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  5. Li Y, et al. 2022. J Oncol. 2022:4544773. PubMed
  6. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  7. Sano Y, et al. 2023. Anticancer Res. 43:547. PubMed
  8. Pušnik J, et al. 2023. Nat Commun. 14:572. PubMed
  9. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  10. Loos C, et al. 2023. NPJ Vaccines. 8:34. PubMed
  11. Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed
  12. Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed
  13. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  14. Bilich T, et al. 2021. Cancer Discov. 11:1982. PubMed
  15. Ali A, et al. 2020. J Infect Dis. 222:853. PubMed
  16. Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed
  17. Peng Q, et al. 2022. EBioMedicine. 77:103904. PubMed
  18. Bauer J, et al. 2022. Nat Commun. 13:6401. PubMed
  19. Das J, et al. 2021. Med (N Y). 2:1269. PubMed
  20. Lübke M, et al. 2020. J Exp Med. 217:00:00. PubMed
  21. Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed
  22. Rice TF, et al. 2021. EBioMedicine. 72:103612. PubMed
  23. Heitmann JS, et al. 2021. Nature. Online ahead of print. PubMed
  24. Minassian AM, et al. 2021. Med (N Y). 2:701. PubMed
  25. Bilich T, et al. 2021. Science Translational Medicine. 13(590):. PubMed
  26. Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
  27. Such L, et al. 2020. J Clin Invest. 4266:130. PubMed
  28. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  29. Grace PS, et al. 2021. Front Immunol. 12:679973. PubMed
  30. Xu C, et al. 2020. Methods Mol Biol. 2108:159. PubMed
  31. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  32. Duque JSR, et al. 2022. Nat Commun. 13:3700. PubMed
  33. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  34. Atyeo C, et al. 2022. Nat Commun. 13:3571. PubMed
  35. Crump NT, et al. 2021. Cell Reports. 35(6):109101. PubMed
  36. Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed
  37. Niu M, et al. 2021. Cell Reports. 36(8):109611. PubMed
  38. Luo Z, et al. 2021. Int J Mol Sci. 22:. PubMed
  39. Jeong HO, et al. 2022. iScience. 25:105358. PubMed
  40. Li L, et al. 2022. JCI Insight. 7:. PubMed
  41. Cimen Bozkus C, et al. 2021. STAR Protoc. 2:100758. PubMed
RRID
AB_315439 (BioLegend Cat. No. 506506)
AB_315439 (BioLegend Cat. No. 506507)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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