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PE anti-mouse CD135 Antibody

Pricing & Availability
Clone
A2F10 (See other available formats)
Regulatory Status
RUO
Other Names
Flk-2, Flt3, Ly-72
Isotype
Rat IgG2a, κ
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Product Citations
publications
A_A2F10_PE_102309
Bone marrow cells from C57BL/6 mouse stained with A2F10 PE and CD45R/B220 (RA3-6B2) FITC
  • A_A2F10_PE_102309
    Bone marrow cells from C57BL/6 mouse stained with A2F10 PE and CD45R/B220 (RA3-6B2) FITC
  • B_A2F10_PE_102309
    Bone marrow cells from C57BL/6 mouse stained with rat IgG2a, k PE and CD45R/B220 (RA3-6B2) FITC
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135305 50 µg £81
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135306 200 µg £230
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Description

CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Flt3 transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Sergejeva S, et al. 2004. Blood 103:1270.
  2. Auffray C, et al. 2009. J. Exp. Med. 206:595.
  3. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
Product Citations
  1. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  2. Turecamo SE, et al. 2018. Matrix Biol. 67:01:00. PubMed
  3. Delbridge AR, et al. 2017. Cell Death Dis. 8:e2914. PubMed
  4. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  5. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  6. Safi F 2023. STAR Protocols. 4(1):101965. PubMed
  7. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  8. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  9. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  10. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  11. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  12. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  13. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  14. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  15. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  16. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  17. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  18. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  19. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  20. Carpenter RS, et al. 2020. Nat Commun. 3.029166667. PubMed
  21. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  22. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  23. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  24. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  25. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  26. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  27. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  28. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  29. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  30. Qian J, et al. 2021. Int J Mol Sci. 22:. PubMed
  31. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  32. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  33. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  34. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  35. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  36. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  37. Sabnis A, et al. 2009. PLoS One. 7:e59. PubMed
  38. Kim SK, et al. 2018. Genes Dev. 32:849. PubMed
  39. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  40. Viaud M, et al. 2020. Cell Reports. 30(10):3397-3410. PubMed
  41. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  42. Jackson JT, et al. 2018. Blood Adv. 2:347. PubMed
  43. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  44. Dingler FA, et al. 2020. Mol Cell. . PubMed
  45. Giotopoulos G, et al. 2015. J Exp Med. 212: 1551-1569. PubMed
  46. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  47. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_1877217 (BioLegend Cat. No. 135305)
AB_1877217 (BioLegend Cat. No. 135306)

Antigen Details

Structure
A 135-150 kD molecule belonging to the tyrosine kinase receptor family.
Distribution

Expressed on early B lymphoid lineage cells in juvenile and adult bone marrow, primitive myeloid progenitors within the BM CD34+ cell population.

Function
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells.
Ligand/Receptor
Flk-2/FLT3 ligand
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Waskow C, et al.Nat. Immunol. 9:676
2. Veiby OP, et al. 1996. Blood 88(4):1256
3. Veiby OP, et al. 1996. J. Immunol. 157(7):2953
4. Mattews W, et al. 1991. Cell. 65(7):1143
5. Hannum C, et al. 1994. Nature 368(2):643
6. Ogawa M, et al. 1998. Exp Hematol. 26(6):478

Gene ID
14255 View all products for this Gene ID
UniProt
View information about CD135 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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