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PE anti-mouse CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
MIH7 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1), B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1
Isotype
Rat IgG2a, λ
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Product Citations
publications
MIH7_PE_CD274_Antibody_032818
C57BL/6 mouse splenocytes were stained with CD274 (clone MIH7, filled histogram) PE, or rat IgG2b, κ PE isotype control (open histogram).
  • MIH7_PE_CD274_Antibody_032818
    C57BL/6 mouse splenocytes were stained with CD274 (clone MIH7, filled histogram) PE, or rat IgG2b, κ PE isotype control (open histogram).
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155403 25 µg £63
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155404 100 µg £172
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Description

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-L1 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The antibody MIH7 does not block other CD274 antibodies: clone MIH6 and 10F.9G2.

Additional reported applications (for the relevant formats) include: Blocking (Block)

Application References

(PubMed link indicates BioLegend citation)
  1. Sharma MD, et al. 2007. J Clin Invest. 117:2570-82. (Block)
  2. Sharma MD, et al. 2015. Sci Adv. 1:e1500845. (Block)
Product Citations
  1. Fox DB, et al. 2023. NPJ Precis Oncol. 7:25. PubMed
  2. Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed
  3. Kalbasi A, et al. 2020. Sci Transl Med. :12. PubMed
  4. Wu G, et al. 2022. Wellcome Open Res. 6:134. PubMed
  5. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  6. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
RRID
AB_2728222 (BioLegend Cat. No. 155403)
AB_2728222 (BioLegend Cat. No. 155404)

Antigen Details

Structure
Type 1 transmembrane protein, member of the B7 family, 40kD.
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Function
CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
Ligand/Receptor
PD-1 (CD279)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, T cells
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Dorand RD. 2016. Science. 353:399
  2. Khan AR, et al. 2015. Nat Commun. 6:5997
  3. Kiyasu J, et al. 2015. Blood. 126:2193
  4. Herold M, et al. 2015 J Immunol. 195:3584
  5. Buddhisa S, et al. 2015. J Immunol. 194:4413
Gene ID
60533 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03/29/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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