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PE/Cyanine7 anti-human IFN-γ Antibody

Pricing & Availability
Clone
4S.B3 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
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Product Citations
publications
4S-B3_PECyanine7_IFN-y_Antibody_1_061324
PMA + ionomycin-stimulated (6 hours, in the presence of Brefeldin A) human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) FITC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IFN-γ (clone 4S.B3) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
  • 4S-B3_PECyanine7_IFN-y_Antibody_1_061324
    PMA + ionomycin-stimulated (6 hours, in the presence of Brefeldin A) human peripheral blood mononuclear cells were surface stained with anti-human CD3 (clone UCHT1) FITC. Cells were then fixed, permeabilized, and intracellularly stained with anti-human IFN-γ (clone 4S.B3) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
Compare all formats See PE/Cyanine7 spectral data
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502527 25 tests £97
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502528 100 tests £221
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified, native human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
  2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
  3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
  4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
  5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
  6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
  8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)
Product Citations
  1. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  2. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  3. Wu X, et al. 2023. iScience. 26:106559. PubMed
  4. Donadei C, et al. 2023. J Clin Med. 12:. PubMed
  5. Imai H, et al. 2023. iScience. 26:106822. PubMed
  6. Fuhrman C, et al. 2015. J Immunol. 195: 145 - 155. PubMed
  7. Luo X, et al. 2020. Front Immunol. 11:623. PubMed
  8. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  9. Witkowski M, et al. 2021. Nature. 600:295. PubMed
  10. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  11. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  12. Kagoya Y, et al. 2018. Nat Med. 24:352. PubMed
  13. Brembilla N, et al. 2011. PLoS One. 15:18741. PubMed
  14. Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed
  15. Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
  16. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  17. Walk J, et al. 2019. Nat Commun. 10:874. PubMed
  18. Bastiaens G, et al. 2016. Am J Trop Med Hyg. 94: 663 - 673. PubMed
  19. Jiang J, et al. 2017. Arthritis Res Ther. 10.1186/s13075-017-1261-9. PubMed
  20. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  21. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  22. Lim D, et al. 2014. J Immunol. 193:5065. PubMed
  23. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  24. Leng T, et al. 2019. Cell Rep. 28:3077. PubMed
  25. Jiang B, et al. 2014. Cell Immunol. 290:10. PubMed
  26. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  27. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  28. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  29. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-11056-9. PubMed
  30. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  31. Ferreira-Gomes M, et al. 2021. Nat Commun. 12:1961. PubMed
  32. Trivedi S, et al. 2020. Elife. 9:00. PubMed
RRID
AB_2123323 (BioLegend Cat. No. 502527)
AB_2123323 (BioLegend Cat. No. 502528)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All IFN-γ Reagents Request Custom Conjugation
Description Clone Applications
PE anti-human IFN-γ 4S.B3 ICFC
APC anti-human IFN-γ 4S.B3 ICFC
FITC anti-human IFN-γ 4S.B3 ICFC
Biotin anti-human IFN-γ 4S.B3 ELISA Detection,ELISPOT Detection,ICFC
Purified anti-human IFN-γ 4S.B3 ELISA,WB,ICFC
Alexa Fluor® 488 anti-human IFN-γ 4S.B3 ICFC
Alexa Fluor® 647 anti-human IFN-γ 4S.B3 ICFC
Alexa Fluor® 700 anti-human IFN-γ 4S.B3 ICFC
Pacific Blue™ anti-human IFN-γ 4S.B3 ICFC
PerCP/Cyanine5.5 anti-human IFN-γ 4S.B3 ICFC
APC/Cyanine7 anti-human IFN-γ 4S.B3 ICFC
PE/Cyanine7 anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 421™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 570™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 605™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 650™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 711™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 785™ anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 510™ anti-human IFN-γ 4S.B3 ICFC
PE/Dazzle™ 594 anti-human IFN-γ 4S.B3 ICFC
APC/Fire™ 750 anti-human IFN-γ 4S.B3 ICFC
PerCP anti-human IFN-γ 4S.B3 ICFC
Brilliant Violet 750™ anti-human IFN-γ 4S.B3 ICFC
KIRAVIA Blue 520™ anti-human IFN-γ Antibody 4S.B3 ICFC
Spark NIR™ 685 anti-human IFN-γ Antibody 4S.B3 ICFC
APC anti-human IFN-γ 4S.B3 ICFC
Spark Blue™ 515 anti-human IFN-γ 4S.B3 ICFC
Spark UV™ 387 anti-human IFN-γ 4S.B3 ICFC
Spark PLUS UV395™ anti-human IFN-γ 4S.B3 ICFC
PE anti-human IFN-γ 4S.B3 ICFC
GMP APC anti-human IFN-γ 4S.B3 ICFC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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