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PerCP/Cyanine5.5 anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
HCD14_PerCPCyanine55_CD14_Antibody_082218
Human peripheral blood monocytes were stained with CD14 (Clone HCD14) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
  • HCD14_PerCPCyanine55_CD14_Antibody_082218
    Human peripheral blood monocytes were stained with CD14 (Clone HCD14) PerCP/Cyanine5.5 (filled histogram), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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325621 25 tests £121
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325622 100 tests £262
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD14 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Kenney DJ, et al. 2022. Cell Rep. 39:110714. PubMed
  2. Lertjuthaporn S, et al. 2022. Pathogens. 11: . PubMed
  3. Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed
  4. Barnes CO, et al. 2022. Sci Adv. 8:eabp8155. PubMed
  5. Porbahaie M, et al. 2023. PLoS One. 18:e0279626. PubMed
  6. Sposito B, et al. 2021. Cell. 184:4953. PubMed
  7. Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed
  8. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  9. Jacobs ME, et al. 2021. Immunother Adv. 1:ltaa004. PubMed
  10. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  11. Ma L, et al. 2021. Clinical Cancer Research. 27(6):1778-1791. PubMed
  12. Antonucci L, et al. 2020. Journal of Immunology Research. 3257.929861. PubMed
  13. Fang W,et al. 2017. PLoS One. 10.1371/journal.pone.0187088. PubMed
  14. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  15. Washburn ML, et al. 2019. J Immunol. 203:1897. PubMed
  16. Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed
  17. Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
  18. Patterson BK, et al. 2022. Front Immunol. 12:746021. PubMed
  19. Jost M, et al. 2021. eLife. 10:00. PubMed
  20. Singh M, et al. 2019. Nat Commun. 10:3120. PubMed
  21. Bsat M, et al. 2015. J Leukoc Biol. 98: 671 - 681. PubMed
  22. Souriant S, et al. 2019. Cell Rep. 26:3586. PubMed
  23. Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
  24. Mendoza-Cabrera MI, et al. 2019. Innate Immun. :1753425919864658. PubMed
  25. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  26. Huber JE, et al. 2020. J Immunol. 204:1101. PubMed
  27. Madsen NH, et al. 2021. Pathogens. 10:. PubMed
  28. Schoofs T et al. 2019. Immunity. 50(6):1513-1529 . PubMed
  29. Genoula M, et al. 2018. Front Immunol. 9:459. PubMed
  30. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  31. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  32. Adhikaree J, et al. 2019. Oncoimmunology. 8:1593803. PubMed
  33. Nielsen M, et al. 2015. PLoS One. 10:121516. PubMed
  34. Georgouli M et al. 2019. Cell. 176(4):757-774 . PubMed
  35. Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed
  36. Schilling B, et al. 2013. Int J Cancer. 133:1653. PubMed
  37. Brittan M, et al. 2012. Eur Respir J. 40:206. PubMed
  38. Kotturi M, et al. 2011. J Virol. 85:11770. PubMed
  39. van Beek JJP, et al. 2020. Cell Reports. 30(4):1027-1038.e4.. PubMed
RRID
AB_893250 (BioLegend Cat. No. 325621)
AB_893250 (BioLegend Cat. No. 325622)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 08/22/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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