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PerCP/Cyanine5.5 anti-human IgM Antibody

Pricing & Availability
Clone
MHM-88 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG1, κ
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Product Citations
publications
MHM-88_PerCPCyanine55_IgM_Antibody_080818
Overnight cultured human peripheral blood lymphocytes were stained with CD19 APC and anti-human IgM (clone MHM-88) PerCP/Cyanine5.5 (left), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
  • MHM-88_PerCPCyanine55_IgM_Antibody_080818
    Overnight cultured human peripheral blood lymphocytes were stained with CD19 APC and anti-human IgM (clone MHM-88) PerCP/Cyanine5.5 (left), or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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314511 25 tests £121
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314512 100 tests £262
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations
  1. Carre C, et al. 2021. iScience. 24:102970. PubMed
  2. Goel RR, et al. 2022. Cell. 185:1875. PubMed
  3. Arunachalam PS, et al. 2023. J Clin Invest. 133:. PubMed
  4. Dou Y, et al. 2023. Antib Ther. 6:76. PubMed
  5. Nunes-Santos CJ, et al. 2023. Nat Commun. 14:3708. PubMed
  6. Lagou V et al. 2018. Cell reports. 25(3):798-810 . PubMed
  7. Sangesland M, et al. 2020. Immunity. 51(4):735-749. PubMed
  8. Català-Moll F, et al. 2021. Nucleic Acids Res. 49:5057. PubMed
  9. Yang Z, et al. 2018. Front Immunol. 9:2613. PubMed
  10. van der Burg M, et al. 2019. Front Immunol. 10:246. PubMed
  11. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  12. Kohn L, et al. 2014. J Immunol. 192:5050. PubMed
  13. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  14. Aggarwal C, et al. 2021. J Virol. 95:e0061021. PubMed
  15. Flint S, et al. 2016. Haematologica. 101: 698 - 706. PubMed
  16. van der Velden V, et al. 2017. J Immunol Methods. 10.1016/j.jim.2017.03.011. PubMed
  17. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  18. Rother M, et al. 2016. J Immunol. 197: 441 - 448. PubMed
  19. Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
  20. van Dongen JJM, et al. 2019. Front Immunol. 10:1271. PubMed
RRID
AB_2076098 (BioLegend Cat. No. 314511)
AB_2076098 (BioLegend Cat. No. 314512)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
3507 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All IgM Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IgM MHM-88 FC,ELISA
PE anti-human IgM MHM-88 FC
Biotin anti-human IgM MHM-88 FC,ELISA
FITC anti-human IgM MHM-88 FC
APC anti-human IgM MHM-88 FC
PerCP/Cyanine5.5 anti-human IgM MHM-88 FC
Pacific Blue™ anti-human IgM MHM-88 FC
Brilliant Violet 421™ anti-human IgM MHM-88 FC
APC/Cyanine7 anti-human IgM MHM-88 FC
Brilliant Violet 570™ anti-human IgM MHM-88 FC
Brilliant Violet 510™ anti-human IgM MHM-88 FC
Brilliant Violet 605™ anti-human IgM MHM-88 FC
Brilliant Violet 650™ anti-human IgM MHM-88 FC
Purified anti-human IgM (Maxpar® Ready) MHM-88 FC,CyTOF®
PE/Dazzle™ 594 anti-human IgM MHM-88 FC
PE/Cyanine7 anti-human IgM MHM-88 FC
Alexa Fluor® 488 anti-human IgM MHM-88 FC
Alexa Fluor® 647 anti-human IgM MHM-88 FC
Alexa Fluor® 700 anti-human IgM MHM-88 FC
Brilliant Violet 711™ anti-human IgM MHM-88 FC
TotalSeq™-A0136 anti-human IgM MHM-88 PG
TotalSeq™-C0136 anti-human IgM MHM-88 PG
Brilliant Violet 785™ anti-human IgM MHM-88 FC
APC/Fire™ 750 anti-human IgM MHM-88 FC
TotalSeq™-B0136 anti-human IgM MHM-88 PG
Spark Violet™ 423 anti-human IgM Antibody MHM-88 FC
TotalSeq™-D0136 anti-human IgM MHM-88 PG
Spark Blue™ 550 anti-human IgM MHM-88 FC
Spark Blue™ 515 anti-human IgM MHM-88 FC
PE/Fire™ 700 anti-human IgM MHM-88 FC
Spark YG™ 593 anti-human IgM MHM-88 FC
PE/Fire™ 640 anti-human IgM MHM-88 FC
Spark Red™ 718 anti-human IgM (Flexi-Fluor™) MHM-88 FC
APC anti-human IgM MHM-88 FC
PE anti-human IgM MHM-88 FC
Brilliant Violet 750™ anti-human IgM MHM-88 FC
Go To Top Version: 4    Revision Date: 08/08/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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