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Purified anti-α-Synuclein, 117-122 Antibody

Pricing & Availability
Clone
A15126D (See other available formats)
Regulatory Status
RUO
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_A15126D_PURE_alphaSynuclein_Antibody_1_WB_011717
Western blot of anti-α-Synuclein antibody (clone A15126D) and isotype-matched IgG2b control, binding to endogenous and recombinant human α-synuclein. Lane 1: 50 ng of recombinant human α-synuclein; Lane 2: 50 ng of recombinant C-terminally truncated human α-synuclein (1-122). Lane 3: 20 µg of normal human brain lysate. Blots were incubated with 1 µg/ml of each primary antibody at room temperature for two hours, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enhanced chemiluminescence was used as the detection system.
  • 1_A15126D_PURE_alphaSynuclein_Antibody_1_WB_011717
    Western blot of anti-α-Synuclein antibody (clone A15126D) and isotype-matched IgG2b control, binding to endogenous and recombinant human α-synuclein. Lane 1: 50 ng of recombinant human α-synuclein; Lane 2: 50 ng of recombinant C-terminally truncated human α-synuclein (1-122). Lane 3: 20 µg of normal human brain lysate. Blots were incubated with 1 µg/ml of each primary antibody at room temperature for two hours, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enhanced chemiluminescence was used as the detection system.
  • 2_A15126D_PURE_alphaSynuclein_Antibody_1_051618
    IHC staining of purified anti-α-Synuclein, 117-122 antibody (clone A15126D) on formalin-fixed paraffin-embedded human Parkinson's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 5 µg/ml of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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848601 25 µg £61
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848602 100 µg £150
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Description

α-synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including  the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms include, a-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.0 - 10 µg/ml. For Direct ELISA, the suggested use of this reagent is 20 ng/ml - 3.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not cross-react with ß- or ?-synucleins using Western blotting or ELISA as detection method.

RRID
AB_2650693 (BioLegend Cat. No. 848601)
AB_2650693 (BioLegend Cat. No. 848602)

Antigen Details

Structure
α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution

Tissue sources: primarily nervous system, but lower expression in other tissues.
Distribution: cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Antigen References

1. Mor DE, et al. 2016. Neurobiol. Dis. 88:66.
2. Jucker M, Walker LC. 2013. Nature. 501:45.
3. Bartels T, et al. 2011. Nature. 477(7362):107.
4. Devine MJ, et al. 2011. Mov. Disord. 26:2160. 

Gene ID
6622 View all products for this Gene ID
UniProt
View information about alpha-Synuclein 117-122 on UniProt.org
Go To Top Version: 2    Revision Date: 01/17/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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