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Purified anti-ERCC1 Antibody

Pricing & Availability
Clone
W20045D (See other available formats)
Regulatory Status
RUO
Other Names
Excision Repair Cross-Complementation Group 1, ERCC excision repair 1, endonuclease non-catalytic subunit, RAD10
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20045D_PURE_ERCC1_Antibody_1_090921.png
Whole cell extracts (15 µg total protein) from HeLa, MCF7, THP-1, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL (1:2000 dilution) of purified anti-ERCC1 antibody (clone W20045D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • W20045D_PURE_ERCC1_Antibody_1_090921.png
    Whole cell extracts (15 µg total protein) from HeLa, MCF7, THP-1, and NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL (1:2000 dilution) of purified anti-ERCC1 antibody (clone W20045D) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker.
  • W20045D_PURE_ERCC1_Antibody_2_090921.png
    ICC staining using purified anti-ERCC1 antibody (clone W20045D) on HeLa cells. The cells were fixed and permeabilized with 100% methanol, and blocked with 5% FBS for 1 hour. The cells were then stained with either no primary antibody (panel A) or 0.625 µg/mL of the primary antibody (panel B), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 anti-goat anti-rat IgG antibody (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the images were captured with a 60X objective.
  • W20045D_PURE_ERCC1_Antibody_3_090921.png
    IHC staining of anti-ERCC1 antibody (clone W20045D) on formalin-fixed paraffin-embedded human prostate cancer (A, positive control) and normal prostate (B, negative control) tissues. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissues were incubated with 5 µg/mL of anti-ERCC1 antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 μm.
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947501 25 µg £81
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947502 100 µg £201
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Description

ERCC1, also referred to as Excision Repair Cross-Complementation Group 1, is a component of the nucleotide excision repair pathway. ERCC1 forms a heterodimer with XPF endonuclease to catalyze the 5’ incision in the process of excising a DNA lesion. ERCC1 also works with SLX4 in homology direct repair of double strand breaks and in the repair of crosslinks. Mutations in ERCC1 play a role in carcinogenesis due to genome instability, and elevated expression of ERCC1 is predictive of resistance to platinum-based chemotherapies in multiple types of cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human ERCC1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.625 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W20045D detects multiple ERCC1 isoforms by western blot.

Clone W20045D was tested for ICC using HeLa cells fixed with methanol or 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. All three methods were compatible with ERCC1 staining.

RRID
AB_2894534 (BioLegend Cat. No. 947501)
AB_2894534 (BioLegend Cat. No. 947502)

Antigen Details

Structure
ERCC1 is a 297 amino acid protein with an expected molecular weight of 33 kD. Four isoforms between 25 and 36 kD have been described.
Distribution

Ubiquitously expressed/ Nucleus

Function
DNA damage repair
Biology Area
Cell Biology, DNA Repair/Replication
Molecular Family
Enzymes and Regulators
Antigen References
  1. Friboulet, et al. 2013. Cell Cycle. 12:3298                                                            
  2. Kashiyama, et al. 2013. J Hum Genet. 92:807                                                    
  3. Wan J, et al. 2017. Canc Invest. 35:85                                                        
  4. Faridounnia, et al. 2018. Molecules. 23:3205                                                                 
Gene ID
2067 View all products for this Gene ID
UniProt
View information about ERCC1 on UniProt.org
Go To Top Version: 1    Revision Date: 09/09/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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