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Purified anti-human CD172a/b (SIRPα/β) Antibody

Pricing & Availability
Clone
SE5A5 (See other available formats)
Regulatory Status
RUO
Other Names
SIRPa, SIRPb, SIRPalpha/beta, BIT, SHPS1, MFR, P84, PTPNS1, CD172 antigen-like family member A, CD172 antigen-like family member B
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SE5A5_Pure_111407
Human peripheral blood monocytes stained with purified SE5A5, followed by anti-mouse-IgG FITC
  • SE5A5_Pure_111407
    Human peripheral blood monocytes stained with purified SE5A5, followed by anti-mouse-IgG FITC
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323801 25 µg £45
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323802 100 µg £74
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Description

CD172a, also known as signal-regulatory protein α (SIRPα), src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, and P84, is a 75-110 kD transmembrane glycoprotein involved in receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily and is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion, and phagocytosis. CD47 (IAP) is the extracellular ligand for CD172a. SIRPα was recently demonstrated to be a specifc marker for cardiomyocytes derived from human pluripotent stem cells2.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NIH-3T3/hu-SIRPα cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone SE5A5 recognizes a common epitope on SIRPa (90 kD) and SIRPß (50 kD)3. A high degree of homology has been found between SIRP family isoforms alpha and beta at the level of extracellular domains. Consequently, many anti SIRP antibody clones, such as SE5A5, have been reported to cross react with several SIRP isoforms1,4,5. It reacts with CD172a and has weak cross-reaction with CD172b. This antibody is able to block the binding of SIRPa (SIRPa1 and SIRPa2) to CD471,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Seiffert M, et al. 1999. Blood 94:3633.
  2. Dubois NC, et al. 2011. Nat. Biotechnol. 29:1011.
  3. Barros MM, et al. 2009. Transfusion 49:154.
  4. Liu Y, et al. 2005. J. Biol. Chem. 280:36132.
  5. Barclay AN. 2009. Curr. Opin. Immunol. 21:47.
  6. Florian S, et al. 2005. J. Leukoc. Biol. 77:984.
Product Citations
  1. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  2. Sim J, et al. 2019. MAbs. 11:1036. PubMed
  3. Tal MC, et al. 2020. MBio. 11:00. PubMed
  4. Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
  5. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  6. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  7. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  8. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  9. Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID
AB_830700 (BioLegend Cat. No. 323801)
AB_830700 (BioLegend Cat. No. 323802)

Antigen Details

Structure
75-110 kD glycoprotein , signal-regulatorey-protein family, Ig superfamily
Distribution

Monocytes/macrophages, granulocytes, dendritic cells, neurons, cardiomyocytes

Function
Negative regulation of leukocyte adhesion and transmigration, macrophage fusion, phagocytosis
Ligand/Receptor
CD47
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Seiffert M, et al. 1999. Blood 94:3633.
2. Seiffert M, et al. 2001. Blood 97:2741.
3. Timms JF, et al. 1998. Mol. Cell Biol. 18:3838.
4. Barclay AN and Brown MH. 2006. Nat. Rev. Immnuol. 6:457.

Gene ID
140885 View all products for this Gene ID
UniProt
View information about CD172a/b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08/31/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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