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Purified anti-human FcεRIα Antibody

Pricing & Availability
Clone
AER-37 (CRA-1) (See other available formats)
Regulatory Status
RUO
Other Names
FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor
Isotype
Mouse IgG2b, κ
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Product Citations
publications
AER-37_Pure_021009.jpg
Human peripheral blood lymphocytes stained with purified AER-37, followed by anti-mouse IgG FITC
  • AER-37_Pure_021009.jpg
    Human peripheral blood lymphocytes stained with purified AER-37, followed by anti-mouse IgG FITC
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334602 100 µg £141
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Description

High affinity IgE receptor (FcεRI) plays a key role in IgE-mediated allergic immune response. FcεRI is a tetrameric receptor complex, which is composed of one α-subunit (FcεRIα), one β-subunit, and two γ-subunits. FcεRIα directly binds IgE with high affinity, while the β- and γ-chains are responsible for mediating intracellular signals. FcεRIα is a 50 kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FcεRIα is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone AER-37 (CRA-1) has been reported to bind the receptor even in the presence of IgE.4

Application References

(PubMed link indicates BioLegend citation)
  1. Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
  2. Suzukawa M, et al. 2005. Int. Immunol. 17:1249.
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
Product Citations
  1. Bawazir M, et al. 2022. Front Immunol. 13:1033794. PubMed
  2. Camiolo MJ, et al. 2021. Cell Reports. 35(2):108974. PubMed
  3. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  4. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  5. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  6. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  7. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
RRID
AB_1227649 (BioLegend Cat. No. 334602)

Antigen Details

Structure
Ig superfamily, 50 kD
Distribution

Mast cells, basophils, cutaneuos Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients

Function
Bind IgE, trigger IgE-mediated allergic response
Ligand/Receptor
IgE
Cell Type
Basophils, Dendritic cells, Eosinophils, Langerhans cells, Mast cells, Monocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Fc Receptors
Antigen References

1. Riske F, et al. 1991. J. Biol. Chem. 266:11245
2. Gounni AS, et al. 2001. FASEB J. 15:940.
3. Maurer D, et al. 1996. J. Immunol. 157:607
4. Maurer d, et al. 1994. J. Exp. Med. 179:745
5. Campbell AM, et al. 1998. Am. J. Respir. Cell Mol. Biol. 19:92.

Gene ID
2205 View all products for this Gene ID
UniProt
View information about FcepsilonRIalpha on UniProt.org
Go To Top Version: 3    Revision Date: 08/16/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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