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Purified anti-IKKα Antibody

Pricing & Availability
Clone
W16228A (See other available formats)
Regulatory Status
RUO
Other Names
Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, Transcription factor 16 TCF-16
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16228A_PURE_IKKalpha_Antibody_082917
Total lysates (15 µg protein) from HeLa , HCT116 and NIH3T3 cell lysates were resolved by 4-20% Tris-Glycine electrophoresis, transferred to nitrocellulose, and probed with 1:250 (2 µg/mL) diluted purified anti-IKKα antibody (clone W16228A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (Cat# 405405). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat# 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W16228A_PURE_IKKalpha_Antibody_082917
    Total lysates (15 µg protein) from HeLa , HCT116 and NIH3T3 cell lysates were resolved by 4-20% Tris-Glycine electrophoresis, transferred to nitrocellulose, and probed with 1:250 (2 µg/mL) diluted purified anti-IKKα antibody (clone W16228A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (Cat# 405405). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat# 643807) was used as a loading control (lower). Lane M: MW ladder.
Cat # Size Price Quantity Check Availability Save
621401 25 µg £70
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621402 100 µg £174
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Description

The inhibitor of nuclear factor-κB kinase-α (IKKα), also known as CHUK, is a catalytic subunit of the IκB kinase (IKK) complex, which is involved in the regulation of the NF-κB signaling pathway. The IKK complex consists of one regulatory subunit (IKKγ/NEMO) and two catalytic subunits (IKKα and IKKβ). Upon activation by external stimuli or pathogen infection, the IKK complex is responsible for the phosphorylation and proteasomal degradation of IκB, followed by translocation of the NF-κB to the nucleus and activation of gene expression. In the noncanonical NF-κB signaling pathway, IKKα has been shown to phosphorylate NF-κB2 (p100), leads to the formation of p52/RelB dimer and activates target genes involved in the development of lymphoid organ, epidermis, and mammary gland. Ikkα deficient mice are perinatally lethal and show impaired development of limb, epidermis, and skin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human IKKα recombinant protein (546-745 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:250-1:2500 diluted (0.2 – 2 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not work for IF microscopy application (in-house tested).

The antibody does not cross react with IKKß based on sequence homology and immunogen sequence.

RRID
AB_2716192 (BioLegend Cat. No. 621401)
AB_2716192 (BioLegend Cat. No. 621402)

Antigen Details

Structure
745 amino acids with a predicted molecular weight of 84.6 kD. Contains an N-terminal kinase domain.
Distribution

Cytoplasm. Nucleus.

Function
IKKα is a catalytic subunit of the IκB kinase (IKK) complex and is involved in the regulation of both canonical and noncanonical NF-κB signaling pathway.
Interaction
IKKα forms the IKK complex with IKKβ and IKKγ. Interacts with TRPC4AP, NALP2, ARRB1, ARRB2, TRPC4AP NLRC5, PIAS1, and MAP3K7.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Hinz M, et al. 2014. EMBO Rep. 15:46.
2. Liu F, et al. 2012. Immunol Rev. 246:239.
3. Shen RR, et al. 2011. Oncogene. 30:631.
4. Perkins ND. 2007. Nat Rev Mol Cell Biol. 8:49.
5. Ohazama A, et al. 2004. Dev Cell. 6:219.
6. Yamamoto Y, et al. 2003. Nature. 423:655.

Gene ID
1147 View all products for this Gene ID
UniProt
View information about IKKalpha on UniProt.org
Go To Top Version: 0    Revision Date: 10/02/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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