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Purified anti-Lamin A/C Antibody

Pricing & Availability
Clone
Poly6135 (See other available formats)
Regulatory Status
RUO
Other Names
LMNA, LMNC, CMD1A, HGPS, LGMD1B, LMN1, LMNL1, MADA, PRO1
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
1_Poly6135_PURE_LaminAslashC_Antibody_1_WB_072921.png
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-Lamin A/C. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
  • 1_Poly6135_PURE_LaminAslashC_Antibody_1_WB_072921.png
    Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-Lamin A/C. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
  • 2_Poly6135_PURE_LaminA_Antibody_2_ICC_080519
    HeLa cells were fixed with 2% PFA for ten minutes, permeabilized with 0.5% Triton X-100 for five minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2 µg/mL anti-Lamin A/C Antibody (clone Poly6135) followed by Alexa Fluor® 594 (red) conjugated goat anti-Rabbit IgG in blocking buffer for two hours at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Cat # Size Price Quantity Check Availability Save
613501 50 µL £61
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613502 200 µL £182
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Description

Lamins A and C are structural components of the lamina, a scaffold of proteins localized to the nuclear inner membrane, and contribute to nuclear membrane dynamics and chromatin structure. Mutations in the LMNA gene have been linked to multiple laminopathy disorders, including Hutchinson-Gilford progeria syndrome (HGPS), an autosomal dominant disorder characterized by the appearance of accelerated and dramatic aging. Patients with HGPS have a mutation in the LMNA gene that results in aberrant splicing and production of the toxic, truncated Lamin A protein progerin that functions in a dominant negative pattern.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant (partial), C-terminal
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, the suggested usage is 1.0 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes

*This antibody weakly cross-reacts with Mouse.

Additional Product Notes
The 50 µL size can be used for approximately 5 Western blots, and the 200 µL size can be used for approximately 20 Western blots.
Application References

(PubMed link indicates BioLegend citation)
  1. Zhang Y, et al. 2007. Mol Cancer Res. 5:909. PubMed
  2. Takezaki S, et al. 2012. J. Immunol. 189:1521. PubMed.
Product Citations
  1. Qian Z, et al. 2010. PLoS One. 6:e1000814. PubMed
  2. Zhang Y, et al. 2007. Mol Cancer Res. 0.839583333. PubMed
  3. Takezaki S, et al. 2012. J Immunol. 189:1521. PubMed
  4. Mori H, et al. 2018. J Dermatol Sci. 90:154. PubMed
  5. Shafiee A,et al. 2017. Sci Rep.. 10.1038/s41598-017-13971-3. PubMed
  6. Sadik A, et al. 2020. Cell. 1252:182. PubMed
RRID
SCR_001134 (BioLegend Cat. No. 613501)
SCR_001134 (BioLegend Cat. No. 613502)

Antigen Details

Structure
Intermediate filament family, farnesyl binding domain, dimer. Isoforms A, AD10, C, approximately 70 kD, 66 kD, and 61 kD, respectively
Distribution

Nucleoplasmic side of inner nuclear membrane

Function
Fibrous component of nuclear lamina, provides framework for nuclear envelope, may interact with chromatin
Interaction
Homodimeric with Lamin C, interacts with LAPs 1A-1C, emerin, Narf, hsMOK2, PKC, SREBP1a, SREBP 1c
Modification
Phosphorylation, Methylation, Farnesylation
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Antigen References

1. Machiels B, et al. 1996. J. Biol. Chem. 271:9249.
2. Barton R, et al. 1999. J. Biol. Chem. 274:30008.
3. Gruenbaum Y, et al. 2000. J. Struct. Biol. 129:313.
4. Stierle V, et al. 2003. Biochemistry 42:4819.

Regulation
Strictly under cell cycle control as seen by disintegration/formation of nuclear envelope in prophase/telophase. Phosphorylation regulates disassembly
Gene ID
4000 View all products for this Gene ID
UniProt
View information about Lamin A/C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 08/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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