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Purified anti-mTOR Antibody

Pricing & Availability
Clone
6H9B10 (See other available formats)
Regulatory Status
RUO
Other Names
FKBP12-rapamycin complex-associated protein 1, FK506 binding protein 12-rapamycin associated protein 1, FRAP1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6H9B10_PURE_mTOR_Antibody_1_WB_071519
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-mTOR Antibody, clone 6H9B10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Lysates were loaded in order of descending mTOR expression (Source: Human Protein Atals).
  • 6H9B10_PURE_mTOR_Antibody_1_WB_071519
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-mTOR Antibody, clone 6H9B10, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Lysates were loaded in order of descending mTOR expression (Source: Human Protein Atals).
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659201 25 µg £61
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659202 100 µg £165
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Description

mTOR (mammalian target of rapamycin) is a 289 kD member of the PI3 kinase family, containing HEAT, FRB, and FATC domains. mTOR is expressed in the nucleus and cytoplasm, and is a target for cell-cycle arrest and the immunosuppressive effects of FKBP12-rapamycin complex. mTOR controls ribosome biogenesis and cell growth, and acts as an ATP sensor. mTOR is repressed by nutrient deprivation and mitochondrial uncoupling. mTOR is the target of PI3K-regulated protein kinase, AKT/PKB (phosphorylated at Thr 2446 and Ser2448). mTOR has also been shown to bind RAPTOR, which negatively regulates mTOR kinase activity, and is also inhibited by tuberin-hamartin tumor suppressor complex. The binding of RAPTOR to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70-alpha.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human mTOR recombinant protein (1186-1385 aa) expressed in E. coli.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Sohrabi Y, et al. 2019. Front Immunol. 9:3155. PubMed
  2. Yang Y, et al. 2022. Nat Commun. 13:5469. PubMed
RRID
AB_2563155 (BioLegend Cat. No. 659201)
AB_2563155 (BioLegend Cat. No. 659202)

Antigen Details

Structure
2549 amino acids encode a protein with predicted MW of 289 kD.
Distribution

Nucleus, Cytoplasm

Function
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex.
Interaction
Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40, and DEPTOR; also part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1, and DEPTOR.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Antigen References

1. Brown EJ, et al. 1994. Nature. 369:756.
2. Dennis P, et al. 2001. Science. 294:1102.
3. Fingar D, et al. 2002. Genes Dev. 16:1472.
4. Nojima H, et al. 2003. J. Biol. Chem. 278:15461.

Gene ID
2475 View all products for this Gene ID
UniProt
View information about mTOR on UniProt.org
Go To Top Version: 4    Revision Date: 07/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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