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Purified anti-NOS1 Antibody

Pricing & Availability
Clone
W19138A (See other available formats)
Regulatory Status
RUO
Other Names
Nitric oxide synthase (NOS1), neuronal NOS (N-NOS)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19138A_PURE_NOS1_Antibody_011922
Whole cell extracts (15 µg total protein) from the indicated tissue lysate or cell line were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-NOS1 (clone W19138A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent Lane M: Molecular weight marker
  • W19138A_PURE_NOS1_Antibody_011922
    Whole cell extracts (15 µg total protein) from the indicated tissue lysate or cell line were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-NOS1 (clone W19138A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent Lane M: Molecular weight marker
  • W19138A_PURE_NOS1_Antibody_1_011922
    IHC staining using purified anti-NOS1 (clone W19138A) on formalin-fixed paraffin-embedded human cerebellum tissue at 10x (left) and 40x (right) objectives. The white box indicates the region of the higher magnification image. The tissue was incubated with 10 μg/mL of the anti-NOS1 antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 Goat anti-rat IgG (red) (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. Scale bar = 50 μM
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602851 25 µg £81
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602852 100 µg £201
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Description

Nitric oxide synthase 1 (NOS1) catalyzes the formation of nitric oxide (NO) from L-arginine. NO signals in neurons intracellularly to regulate metabolic activity and dendrite growth. NO also functions in neurons as a neurotransmitter-like signaling molecule, and in muscle cells, where it is a potent relaxer. High concentrations of NO are cytotoxic, so it is utilized by macrophages as a bactericide/tumoricide, and it has also been implicated in the progression of neurodegenerative disorders like Alzheimer’s.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human NOS1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product was tested and failed for IHC-F in mouse brain.

RRID
AB_2910479 (BioLegend Cat. No. 602851)
AB_2910479 (BioLegend Cat. No. 602852)

Antigen Details

Structure
NOS1 is a 1,434 amino acid protein with a predicted molecular weight of 161 kD.
Distribution

NOS1 is expressed in the cytoplasm of neurons, skeletal and smooth muscle cells, and macrophages.

Function
NOS1 is an enzyme that synthesizes nitric oxide.
Cell Type
Macrophages, Mature Neurons, Neurons
Antigen References
  1. Kourosh-Arami M, et al. 2019. Rev. Neurosci. 31:617-636.
  2. Jin R and Loscalzo J. 2010. J. Blood Med. 1:147-162. 
Gene ID
4842 View all products for this Gene ID
UniProt
View information about NOS1 on UniProt.org
Go To Top Version: 1    Revision Date: 01/19/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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