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Purified anti-Tau Phospho (Thr231) Antibody (Previously Covance catalog# MMS-545P)

Pricing & Availability
Clone
PHF-6 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein tau, PHF-tau, paired helical filament-tau, neurofibrillary tangle protein, microtubule-associated protein tau, isoform 4, G protein beta1/gamma2 subunit-interacting factor 1
Previously
Covance Catalog# MMS-545P
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
PHF-6_PURE_Tau-Phospho-Thr231_Antibody_1_032123
Rat brain whole tissue lysates (15 µg total protein) from 31 days (negative control) and 5 days post-partum animals (positive control) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-Tau phospho (Thr231) (clone PHF-6) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 649204) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • PHF-6_PURE_Tau-Phospho-Thr231_Antibody_1_032123
    Rat brain whole tissue lysates (15 µg total protein) from 31 days (negative control) and 5 days post-partum animals (positive control) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1.0 µg/mL of purified anti-Tau phospho (Thr231) (clone PHF-6) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 649204) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • PHF-6_PURE_Tau-Phospho-Thr231_Antibody_2_040323
    IHC staining of purified anti-Tau Phospho (Thr231) (clone PHF-6) on formalin-fixed paraffin-embedded human Alzheimer's brain tissue. Following antigen retrieval using 88% formic acid, the tissue was blocked and incubated without (Panels A and C) and with (Panels B and D) Lambda Phosphatase Protein overnight at 4°C. The tissue was then incubated without (panels A and B) or with (panel C and D) 5 µg/mL of purified anti-Tau Phospho (Thr231) (clone PHF-6) overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB) (Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective. Scale bar: 50 µm
  • PHF-6_PURE_Tau-Phospho-Thr231_Antibody_3_040323
    Biotinylated Tau phospho (Thr231) (red line) or non-phospho (blue line) peptides were incubated at 2 µg/mL onto 96-well plates coated with NeutrAvidin in 0.1% BSA-PBST overnight at 4°C. Direct ELISA was performed by further incubating the plate with serial dilutions of purified anti-Tau phospho (Thr231) (clone PHF-6) in 0.1% BSA-PBST for 1 hour at room temperature. Bound antibody was detected with HRP Goat anti-mouse IgG (Cat. No. 405306). TMB substrate set (Cat. No. 421101) was used as a detection system. Absorbance was measured at 450 nm.
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828903 25 µL £77
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828901 100 µL £245
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Description

Paired helical filaments (PHFs) are the major building blocks of neurofibrillary lesions in Alzheimer's disease brains and are composed of hyperphosphorylated tau protein. Predominantly expressed in axons, alternatively spliced forms of tau comprise a family of microtubule-associated proteins that normally promote and stabilize the assembly of microtubules. PHF-tau differs from normal tau by its abnormal hyperphosphorylation, which results in decreased binding of tau to microtubules. The decreased affinity of PHF-tau for microtubules, coupled with reduced levels of normal tau, destabilizes microtubules leading to an impairment of axonal transport, neuronal death and the aggregation of PHFs. Therefore, hyperphosphorylation of tau is believed to be a key event in the pathogenesis of Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The PHF-6 antibody was raised using PHF-tau preparations from human brain.
Formulation
Phosphate-buffered solution; no preservatives or carrier proteins.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

The PHF-6 antibody reacts with human tau phosphorylated at Thr 231.

Direct ELISA was done using phospho and non-phospho specific peptides, mainly to confirm specificity. Application has not been verified on tissue/cell lysates.

Application References

(PubMed link indicates BioLegend citation)
  1. Lasagna-Reeves CA, et al. 2012. FASEB J. 26:1946-1959.
  2. Mitchell TW, et al. 2000. J Histochem Cytochem. 48:1627-1637.
  3. Hong M, et al. 1997. J Biol Chem. 272:25326-25332.
  4. Bramblett GT, et al. 1993. Neuron. 10:1089-1099.
Product Citations
  1. Loeffler D, et al. 2015. Exp Gerontol. 67: 15-18. PubMed
RRID
AB_2564924 (BioLegend Cat. No. 828903)
AB_2564924 (BioLegend Cat. No. 828901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Phospho-Proteins, Tau
Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau Phospho Thr231 on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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