Ultra-LEAF Purified anti-human CD64 Antibody, CD64, 10.1

Pricing & Availability

Clone: 10.1 (See other available formats)
Regulatory Status: RUO
Workshop: VI MA36
Other Names: FcγRI, FcR I
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood monocytes were stained with CD64 (clone 10.1) Purified (filled histogram) or Purified Mouse IgG1, κ isotype control (open histogram) followed by anti-mouse IgG FITC

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305047

100 µg

£151

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305048

1 mg

£520

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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, Capuchin Monkey, Chimpanzee, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human rheumatoid synovial fluid cells and fibronectin-purified monocytes.
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
IHC-F, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 10.1 recognizes the EC3 epitope of CD64. While both contain the EC3 domain, in-house testing suggests that clone 10.1 preferentially binds to CD64A (FcγRIA), but not CD64B (FcγRIB). Additional reported applications (for the relevant formats) include: blocking of human IgG3 and murine IgG2a binding to FcγRI^2,5,6,11 and immunohistochemical staining of acetone-fixed frozen tissue sections¹².
RRID: AB_2810456 (BioLegend Cat. No. 305047)
AB_2810456 (BioLegend Cat. No. 305048)

Antigen Details

Structure: Ig superfamily, type I glycoprotein, 72 kD
Distribution: Monocytes, macrophages, dendritic cells, activated granulocytes
Function: Phagocytosis, ADCC
Ligand/Receptor: IgG receptor
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Hulett M, et al. 1994. Adv. Immunol. 57:1.
2. van de Winkel J, et al. 1993. Immunol. Today 14:215.
Gene ID: 2209 View all products for this Gene ID
UniProt: View information about CD64 on UniProt.org

Documentation

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Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?: Yes
Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD64 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-human CD64	10.1	FC
FITC anti-human CD64	10.1	FC
PE anti-human CD64	10.1	FC
Purified anti-human CD64	10.1	FC,IHC-F,Block
Alexa Fluor® 488 anti-human CD64	10.1	FC
Alexa Fluor® 647 anti-human CD64	10.1	FC
APC anti-human CD64	10.1	FC
Pacific Blue™ anti-human CD64	10.1	FC
Brilliant Violet 421™ anti-human CD64	10.1	FC
PE/Cyanine7 anti-human CD64	10.1	FC
PerCP/Cyanine5.5 anti-human CD64	10.1	FC
APC/Cyanine7 anti-human CD64	10.1	FC
Brilliant Violet 510™ anti-human CD64	10.1	FC
Purified anti-human CD64 (Maxpar® Ready)	10.1	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD64	10.1	FC
Brilliant Violet 605™ anti-human CD64	10.1	FC
APC/Fire™ 750 anti-human CD64	10.1	FC
PE anti-human CD64	10.1	FC
PE/Dazzle™ 594 anti-human CD64	10.1	FC
FITC anti-human CD64	10.1	FC
TotalSeq™-A0162 anti-human CD64	10.1	PG
Brilliant Violet 711™ anti-human CD64	10.1	FC
Alexa Fluor® 700 anti-human CD64	10.1	FC
Brilliant Violet 785™ anti-human CD64	10.1	FC
TotalSeq™-C0162 anti-human CD64	10.1	PG
Ultra-LEAF™ Purified anti-human CD64	10.1	FC,IHC-F,Block
TotalSeq™-B0162 anti-human CD64	10.1	PG
TotalSeq™-D0162 anti-human CD64	10.1	PG
GMP PE anti-human CD64	10.1	FC
GMP FITC anti-human CD64	10.1	FC
Brilliant Violet 650™ anti-human CD64	10.1	FC
GMP PE/Dazzle™ 594 anti-human CD64	10.1	FC
PE/Cyanine7 anti-human CD64	10.1	FC
APC/Fire™ 750 anti-human CD64	10.1	FC
PerCP/Cyanine5.5 anti-human CD64	10.1	FC
Pacific Blue™ anti-human CD64	10.1	FC
GMP APC/Fire™ 750 anti-human CD64	10.1	FC
GMP PE/Cyanine7 anti-human CD64	10.1	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 05/28/2019

For Research Use Only. Not for diagnostic or therapeutic use.

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