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Veri-Cells™ CD34 PBMC were stained with CD45 (clone HI30) FITC and CD34 (clone 561) PE. Dot plots are gated on single cells.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 426901 | 10 tests | £81 | ||||
| 426903 | 50 tests | £301 | ||||
The reconstituted Veri-Cells™ CD34 PBMC can be used for immunofluorescent staining and multi-color flow cytometry assays. The cells can be used as controls for stability studies to avoid donor variability. CD34+ population in the Veri-Cell CD34 PBMC co-expresses CD7, CD13 and CD71. In addition to these, the PBMC population in this product has been verified to work with commonly tested cell markers such as CD3, CD4, CD8, CD19, CD20, CD56/16, CD14, CD25, CD69, GrB, Perforin, Foxp3 and Helios.
This product contains human cells, a potentially biohazardous material. Blood used in preparation of these samples was tested and found to be negative, using FDA approved methods, for antibody and nucleic acid testing against Human Immunodeficiency virus (HIV), Hepatitis B and C virus and HTLV (Human T-Lymphotrophic virus I and II), surface antigen for Hepatitis B (HBsAg) virus and syphilis and West Nile Virus. Biological tests are not 100% accurate. Use standard precautions when handling and treat as if the product is capable of transmitting disease. When handling or disposing, follow precautions described in CDC and FDA recommendations and OSHA Bloodborne Pathogen guidelines.
Kit Contents
- Kit Contents
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Cat. No. 426901
- 2 vials of Veri-Cell™ CD34 PBMC (approximately 1 X 106 cells per vial)
- 1 vial of 0.7 ml Veri-Cells™ Buffer A Plus
Cat. No. 426903
- 10 vials of Veri-Cell™ CD34 PBMC (approximately 1 X 106 cells/vial)
- 5 vials of 0.7 ml Veri-Cells™ Buffer A Plus
Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.
Product Details
- Formulation
- Each kit will contain 2 vials of Veri-Cells™ CD34 PBMC and one vial of Veri-Cells™ Buffer A Plus. Each lyophilized vial contains ≥ 1 X 106 cells per vial (5 Tests).
- Preparation
- The Veri-Cells™ CD34 PBMC kit is a mixture of CD34+ KG-1a cells and human PBMCs.
- Storage & Handling
- Store the kit at 2 - 8°C upon recipt. Do not open the sealed pouch until ready to use. The reconstituted cells may be used for up to 5 days if properly stored at 2 - 8°C in the buffer provided.
- Application
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FC - Quality tested
- Recommended Usage
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50 µl per test (after reconstitution with 325 µl of Veri-Cells™ Buffer A Plus)
Once reconstituted, let the cells sit for 15 minutes at room temperature prior to staining.
Veri-Cells™ Reconstitution Buffer A Plus may show precipitation over time, however this is normal and does not affect performance of the buffer. - Application Notes
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Naturally occurring CD34 positive cells have characteristics of human hematopoietic progenitor cells reported in frequencies as low as 0.001% of the peripheral blood cells in healthy subjects. The Veri-Cells™ CD34 PBMC staining control allows the user to verify CD34 antibody binding without the need for artificial enrichment or the collection of very large numbers of events. The targets detectable on Veri-Cells™ PBMC (Cat. Nos. 425001-4) are also present on this product.
The Veri-Cells™ CD34 PBMC Kit can be used as a positive control for surface and intracellular immunofluorescent staining and multi-color flow cytometry assays. It can also be used for the detection of intracellular molecules such as Granzyme B, Perforin, Foxp3 and Helios using BioLegend’s Fixation Buffer (Cat. No. 420801) and Intracellular Staining Permeabilization Wash Buffer (10X) (Cat. No. 421002) or the True Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). For best results, Cell Staining Buffer (Cat. No. 420201) is recommended for any washing steps. Please note, any claims relative to product performance are based on testing with BioLegend reagents and protocols. Deviations from the use of these reagents and procedures have not been assessed and may affect performance.Cell subset
Acceptance Criteria
CD34+CD45+
3-20%
Note on indicated ranges: Percent positive values are based on gates set on unstained cells. The antibodies used for testing were the following: CD34 PE (clone 581) and CD45 FITC (clone HI30). Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. The Certificate of Analysis specific values are provided as a reference. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols.
Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of CD45+/CD34+ cells included within each lot. The percent positive is determined by a CD34 (clone 561) PE vs.CD45 (clone HI30) FITC plot gated on single cells. The Certificate of Analysis specific values are provided only as a reference. Variability in antibodies used, instruments, staining conditions and other factors may influence the end user values. - Additional Product Notes
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Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes.
Antigen Details
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Gene ID
- NA
Related Pages & Pathways
Pathways
Related FAQs
- Is washing required after staining?
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Yes, it is recommended to wash after staining. We recommend using BioLegend's Cell Staining buffer (Cat. No. 420201) for the wash step.
- Can the cells be activated after rehydration?
- No, they cannot be activated.
- Are these cells virus/pathogen free?
- These are tested to be free of HIV, HBV, syphilis, and HCV.
- Are there any RBCs in Veri-cells™?
- There may be a few, but the majority of RBCs are not present in Veri-Cells™ preparations.
- Are these cells viable?
- No they are not.
- Can a constant percentage of each population be expected in each lot?
- Yes, within each lot, cell populations remain constant.
- Are the cells fixed?
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They are treated with our proprietary mixture prior to lyophilization.
- Can they be used to detect intracellular cytokines such as TNF-α or IFN-γ?
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For this, use Veri-Cells™ Activated (Cytokine) PBMC which have been activated with Cell Activation Cocktail (with Brefeldin A) containing PMA and Ionomycin. These have been validated to stain positive for activation-induced cytokines including TNF-α and IFN-γ. Veri-Cells™ PBMC and Veri-Cells™ CD4-Low PBMC will not have detectable TNF-α or IFN-γ.
- How do we QC these cells?
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Each Veri-Cells™ product is tested with a panel of antibodies to ensure expression of these markers falls within the expected range. For more information regarding the exact markers used for each product, please contact technical service.
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