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APC anti-mouse CD40 Antibody

Pricing & Availability
Clone
3/23 (See other available formats)
Regulatory Status
RUO
Other Names
Bp50, TNFRSF5
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
3slash23_APC_081908.jpg
BALB/c splenocytes stained with 3/23 APC
  • 3slash23_APC_081908.jpg
    BALB/c splenocytes stained with 3/23 APC
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Cat # Size Price Quantity Check Availability Save
124611 25 µg 82€
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124612 100 µg 253€
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Description

CD40 is a 48 kD type I transmembrane glycoprotein also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD40 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124627 &124628) with a low endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References
  1. Hasbold J, et al. 1994. Eur. J. Immunol. 24:1835.
  2. Bourgeois C, et al. 2002. Science 297:2060.
Product Citations
  1. Doherty CM, et al. 2022. Parasite Immunol. 44:e12897. PubMed
  2. Motyka NI, et al. 2022. Infect Immun. 90:e0063721. PubMed
  3. Wu R, et al. 2022. Nat Immunol. 23:1536. PubMed
  4. Solier S, et al. 2023. Nature. 617:386. PubMed
  5. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  6. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  7. Chakrabarti J, et al. 2018. Oncotarget. 9:37439. PubMed
  8. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  9. Jiang C, et al. 2020. Cell Reports. 28(5):1307-1322.e8.. PubMed
  10. Polavaram NS, et al. 2021. Bone Res. 9:24. PubMed
  11. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  12. Guo Y, et al. 2022. Nat Commun. 13:6534. PubMed
  13. Farkas A, et al. 2013. J Immunol. 190:3319. PubMed
  14. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
  15. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  16. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  17. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
  18. Grabinger T, et al. 2019. Front Microbiol. 10:1385. PubMed
  19. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  20. Ren S, et al. 2022. Int J Biol Sci. 18:166. PubMed
  21. Mino T, et al. 2019. Nucleic Acids Res. 47:8838. PubMed
  22. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
RRID
AB_1134072 (BioLegend Cat. No. 124611)
AB_1134072 (BioLegend Cat. No. 124612)

Antigen Details

Structure
48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
Distribution

Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.

Function
Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
Ligand/Receptor
CD154 on T cells
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Grewal IS, et al. 1998. Annu Rev Immunol 16:111.

Gene ID
21939 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD40
Specificity Alt (DOES NOT SHOW ON TDS):
CD40
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD40 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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