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Brilliant Violet 421™ anti-human IL-13 Antibody

Pricing & Availability
Clone
JES10-5A2 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-13, NC30
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
JES10dash5A2_BV421_IL13_Antibody_1_042716
TH2 polarized human peripheral blood lymphocytes were surface stained with anti-human CD3 PE then intracellularly stained with IL-13 (Clone JES10-5A2) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • JES10dash5A2_BV421_IL13_Antibody_1_042716
    TH2 polarized human peripheral blood lymphocytes were surface stained with anti-human CD3 PE then intracellularly stained with IL-13 (Clone JES10-5A2) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • JES10dash5A2_BV421_IL13_Antibody_2_042716
Compare all formats See Brilliant Violet 421™ spectral data
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501915 25 tests 141€
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501916 100 tests 304€
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Description

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The JES10-5A2 antibody reacts with human interleukin-13 (IL-13). The JES10-5A2 antibody can neutralize the bioactivity of natural or recombinant IL-13.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed, recombinant human IL-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

ELISA or ELISPOT Capture1,2: The Ultra-LEAF™ Purified JES10-5A2 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5020 antibody (Cat. No. 502001) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture.

Application References
  1. McKenzie, A., et al. 1994. Curr. Prot. Immunol.. John Wiley & Sons. New York. Section 6.18.
  2. Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
  3. Schaerli, P., et al. 2000. J. Exp. Med. 192:1553.
  4. Andersson, J., et al. 1994. Immunology 83:16.
  5. Skansen-Saphir, U., et al. 1994. Eur. J. Immunol. 24:916.
  6. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  7. Gelfand, E. W., et al. 2006. J. Aller. and Clinic. Immunol. 117:577.
  8. Knorr, C., et al. 1999. Am. J. Pathol. 155:2019.
Product Citations
  1. Mashayekhi M, et al. 2022. Prostaglandins Other Lipid Mediat. 158:106604. PubMed
  2. Solforosi L, et al. 2021. J Exp Med. :218. PubMed
  3. Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
  4. Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed
RRID
AB_2616747 (BioLegend Cat. No. 501915)
AB_2616747 (BioLegend Cat. No. 501916)

Antigen Details

Structure
Cytokine; 17 kD (Mammalian)
Bioactivity
Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes
Cell Sources
Activated T cells, mast cells, NK cells
Cell Targets
B cells, monocytes, macrophages, basophils
Receptors
Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497.
3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797.
4. Ochensberger, B., et al. 1996. Blood 88:3028.

Regulation
IL-13Rα2 possible antagonist
Gene ID
3596 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
IL-13
Specificity Alt (DOES NOT SHOW ON TDS):
IL-13
App Abbreviation (DOES NOT SHOW ON TDS):
ICFC
UniProt
View information about IL-13 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All IL-13 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human IL-13 JES10-5A2 ICFC
LEAF™ Purified anti-human IL-13 JES10-5A2 ELISA Capture, ELISPOT Capture, ICFC, IHC, Neut, WB
PE anti-human IL-13 JES10-5A2 ICFC
Purified anti-human IL-13 JES10-5A2 ELISA Capture,IHC,WB
PerCP/Cyanine5.5 anti-human IL-13 JES10-5A2 ICFC
PE/Cyanine7 anti-human IL-13 JES10-5A2 ICFC
Brilliant Violet 421™ anti-human IL-13 JES10-5A2 ICFC
Ultra-LEAF™ Purified anti-human IL-13 JES10-5A2 ELISA Capture,ELISPOT Capture,ICFC,IHC,Neut,WB
Go To Top Version: 1    Revision Date: 04/28/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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