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Purified anti-α-Synuclein, aggregated Antibody

Pricing & Availability
Clone
Syn-F1 (See other available formats)
Regulatory Status
RUO
Other Names
Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Syn-F1_PURE_alpha-synuclein_Antibody_1_IHC_112816
IHC staining of α-Synuclein deposits with anti-α-Synuclein, aggregated antibody (clone Syn-F1) on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using citrate and 0.25% Triton X-100, the tissue was incubated with the primary antibody at 1 µg/mL overnight at 4°C. Biolegend's Ultra-Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • Syn-F1_PURE_alpha-synuclein_Antibody_1_IHC_112816
    IHC staining of α-Synuclein deposits with anti-α-Synuclein, aggregated antibody (clone Syn-F1) on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using citrate and 0.25% Triton X-100, the tissue was incubated with the primary antibody at 1 µg/mL overnight at 4°C. Biolegend's Ultra-Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • Syn-F1_PURE_alpha-synuclein_Antibody_2_DB_112816
    Dot-blot of anti-α-Synuclein antibodies (clones Syn-F1 and 4B12/Synuclein) and isotype-matched IgG1 control, demonstrating their binding to α-synuclein conformers (monomeric and fibrillar). Note that these antibodies do not cross-react with amyloid beta peptide (Aβ1-42). Blots were incubated at room temperature for two hours with 0.5 µg/mL of the primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enha
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Description

α-Synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Aggregated α-synuclein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Dot-Blot - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For Dot Blot, a concentration range of 0.5 - 3.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not cross-react with ß- or ?-synucleins using Dot Blot as detection method.

Application References
  1. Vaikath NN, et al. 2015. Neurobiol Dis. 79:81. (IHC, ELISA Capture, Dot-Blot)

Antigen Details

Structure
α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Distribution

Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.

Cell Sources
Primarily nervous system, but lower expression in other tissues.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
α-Synuclein
Antigen References

1. Mor DE, et al. 2016. Neurobiol Dis. 88:66.
2. Jucker M, Walker LC. 2013. Nature. 501(7465):45.
3. Bartels T, et al. 2011. Nature. 477(7362):107.
4. Devine MJ, et al. 2011. Mov Disord. 26:2160.

Gene ID
6622 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
alpha-Synuclein, aggregated
Specificity Alt (DOES NOT SHOW ON TDS):
α-Synuclein, aggregated
App Abbreviation (DOES NOT SHOW ON TDS):
IHC-P, Dot Blot
UniProt
View information about alpha-Synuclein, aggregated on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/07/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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