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- Regulatory Status
- RUO
- Other Names
- T-cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
- Ave. Rating
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- Product Citations
- publications
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Recombinant human IL-2 induces the proliferation of mouse HT-2 cells in a dose-dependent manner. The ED50 for this effect is 0.3 - 1.5 ng/mL. -
Stability Testing for Recombinant Human IL-2. Recombinant human IL-2 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4°C (Control). The samples were tested for their ability to induce the proliferation of mouse HT-2 cells in a dose-dependent manner. The ED50 for this effect is 0.3 - 1.5 ng/mL.
IL-2 was discovered through its function as a T cell growth factor (TCGF). It plays a pivotal role in immune responses against pathogenic infection. Recognition and binding of foreign antigens by the TCRs stimulate both the secretion of IL-2 and the expression of IL-2Rs on the T cell surface. Subsequently, the IL-2/IL-2R interaction activates the intracellular Ras/Raf/MAPK, JAK/STAT, and PI3K/AKT signaling pathways, and ultimately stimulates the growth, differentiation, and survival of the Ag-selected cytotoxic T cells. Human IL-2 acts on murine and human T cells, and its receptors are shared by other cytokines. IL-2Rα is an IL-2–specific receptor, IL-2Rβ is shared with IL-15 and the γ chain that is a common receptor shared by many cytokines including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21.
Product DetailsProduct Details
- Source
- Human IL-2, amino acid (Ala21-Thr153) (Accession: # P60568) was expressed in E. coli.
- Molecular Mass
- The 133 amino acid recombinant protein has a predicted molecular mass of approximately 15.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 15 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE
- Formulation
- 0.22 µm filtered protein solution is in 0.1% TFA, 10% Acetonitrile
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Recombinant human IL-2 induces the proliferation of mouse HT-2 cells in a dose- dependent manner. The ED50 for this effect is 0.3 - 1.5 ng/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
- Product Citations
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Antigen Details
- Structure
- Monomer
- Distribution
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T cells (CD4+ and CD8+), gamma δ T cells, B cells, dendritic cells, and eosinophils. Mainly activated T cells.
- Function
- Differentiation of Th1 and Th2 cells, inhibition of Th17 and Tfh cell differentiation, regulation of Treg differentiation, T cell proliferation, B cell proliferation, promotes CD8 and NK cell cytolytic activity.
- Interaction
- CD4 T cells, T reg cells, CD8 effector cells, NK cells, B cells
- Ligand/Receptor
- Trimeric receptor: IL-2Rα (CD25), IL-2Rβ (CD122), and γc (CD132) and dimeric receptor:IL-2Rβ (CD122) and γc (CD132)
- Bioactivity
- Recombinant human IL-2 induces the proliferation of mouse HT-2 cells.
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
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- Siegel JP, et al. 1987. Science. 238:75-8.
- Cote-Sierra J, et al. 2004. Proc Natl Acad Sci U S A. 101:3880-5.
- Laurence A, et al. 2007. Immunity. 26:371-81.
- Sakaguchi S, et al. 2008. Cell. 133:775-87.
- Liao W, et al. 2011. Nat Immunol. 12:551-9.
- Ballesteros-Tato A, et al. 2012. Immunity. 36:847-56.
- Liao W, et al. 2013. Immunity. 38:13-25.
- Read KA, et al. 2016. Exp Hematol. 44:799-808.
- Gene ID
- 3558 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- IL-2
- Specificity Alt (DOES NOT SHOW ON TDS):
- IL-2
- App Abbreviation (DOES NOT SHOW ON TDS):
- BA
- UniProt
- View information about IL-2 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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