- Clone
- P84 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- SHPS-1, BIT, P84, PTPNS1, CD172 antigen-like family member A
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
144023 | 25 µg | 109€ | ||||
144024 | 100 µg | 278€ |
CD172a, also known as SIRPα, is a type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. CD172a is expressed by monocytes, macrophages, myeloid cells, and neuronal tissue. The phosphorylation of SIRPα ITIMs induces the recruitment and activation of the tyrosine phosphatases PTPN6 and PTPN11, resulting in the negative regulation of several biological processes. The ligands of CD172a are CD47, SP-A, and SP-D.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse brain membrane protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB - Reported in the literature, not verified in house
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking SIRPa interaction with CD474, in vivo blocking of dendritic cell migration3, enhancing of macrophage phagocytosis2,4, immunohistochemical staining of cerebellum frozen sections1, immunoprecipitation2,4, and spatial biology (IBEX)5,6.
- Additional Product Notes
-
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
- Application References
-
- Comu S, et al. 1997. J. Neurosci. 17:8702. (IHC)
- Gresham HD, et al. 2000. J. Exp. Med. 191:515. (IP)
- Fukunaga A, et al. 2004. J. Immunol. 172:4091. (Block)
- Oldenborg PA, et al. 2000. Science 288:2051. (Block, IP)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2650814 (BioLegend Cat. No. 144023)
AB_2650814 (BioLegend Cat. No. 144024)
Antigen Details
- Structure
- Type I transmembrane protein
- Distribution
-
Monocytes, macrophages, myeloid cells, neuronal tissue
- Function
- Negative regulation of several biological processes
- Interaction
- PTPN6, PTPN11
- Ligand/Receptor
- CD47, SP-A, SP-D
- Cell Type
- Dendritic cells, Macrophages, Monocytes, Neutrophils
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase
- Antigen References
-
1. Zhao XW, et al. 2011. P. Natl. Acad. Sci. USA 108:18342.
2. Verjan-Garcia N, et al. 2011. J. Immunol. 187:2268.
3. Sato-Hashimoto M, et al. 2011. J. Immunol. 187:291.
4. Raymond M, et al. 2010. Eur. J. Immunol. 40:3510. - Gene ID
- 19261 View all products for this Gene ID
- UniProt
- View information about CD172alpha on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
-
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
-
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
-
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
-
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
-
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
FITC anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... Fixed whole mount mouse spleen was stained with FITC CD172a ... -
PE/Cyanine7 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
PerCP/Cyanine5.5 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
PE anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
APC anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
PE/Dazzle™ 594 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
Alexa Fluor® 594 anti-mouse CD172a (SIRPα)
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
APC/Cyanine7 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Alexa Fluor® 488 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... Confocal image of C57BL/6 mouse thymus sample acquired using... Mice were injected subcutaneously with sheep red blood cells... -
Alexa Fluor® 700 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Biotin anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... -
Alexa Fluor® 647 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
APC/Fire™ 750 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... -
TotalSeq™-A0422 anti-mouse CD172a (SIRPα)
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Brilliant Violet 510™ anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
TotalSeq™-C0422 anti-mouse CD172a (SIRPα)
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Ultra-LEAF™ Purified anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with CD11b APC ... -
TotalSeq™-B0422 anti-mouse CD172a (SIRPα)
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PerCP/Fire™ 780 anti-mouse CD172a (SIRPα)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Spark Red™ 718 anti-mouse CD172a (SIRPα) (Flexi-Fluor™)
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