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Alexa Fluor® 488 anti-SPI1 (PU.1) Antibody

Pricing & Availability
Clone
7C2C34 (See other available formats)
Regulatory Status
RUO
Other Names
Spleen focus forming virus (SFFV) proviral integration oncogene, Sfpi1, Transcription factor PU.1, Dis1, PU.1, Dis-1, Sfpi1, Spi-1, Sfpi-1, Tcfpu1, Tfpu.1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
7C2C34_A488_SPI1_Antibody_1_070616
C57BL/6 splenocytes were surface stained with CD19 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were intracellularly stained with SPI1 (clone 7C2C34) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
  • 7C2C34_A488_SPI1_Antibody_1_070616
    C57BL/6 splenocytes were surface stained with CD19 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were intracellularly stained with SPI1 (clone 7C2C34) Alexa Fluor® 488 (top) or rat IgG2a, κ Alexa Fluor® 488 isotype control (bottom).
  • 7C2C34_A488_SPI1_Antibody_2_070616
Compare all formats See Alexa Fluor® 488 spectral data
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681305 25 µg 128€
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681306 100 µg 287€
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Description

SPI1 is a transcription factor that belongs to the E26-transformation-specific (ETS) family and is exclusively expressed in hematopoietic cells. SPI1 regulates cell fate decisions during the differentiation of hematopoietic stem cells, which is crucial for the development of lymphoid and myeloid cell lineages. SPI1-deficient mice lack macrophages, neutrophils, and B lymphocytes, and they die before or shortly after birth. Abnormally regulated expression of SPI1 can lead to developmental defects as well as malignancy. The overexpression of SPI1 blocks erythroid differentiation and inhibits cell death. Mice carrying a mutant SPI1 allele shows decreased SPI1 expression and develops acute myeloid leukaemia (AML), which suggests that SPI1 plays a role in oncogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length SPI1 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

RRID
AB_2629611 (BioLegend Cat. No. 681305)
AB_2629611 (BioLegend Cat. No. 681306)

Antigen Details

Structure
272 amino acids with a predicted molecular weight of 31.3 kD. It contains a C-terminal ETS domain that is responsible for DNA binding.
Distribution

Nucleus.

Function
SPI1 is a transcription factor that is required for the development of lymphoid and myeloid cells.
Interaction
SPI1 interacts with RUNX1, CEBPD, NONO, SPIB, and GFI1.
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Hikami K, et al. 2011. Arthritis Rheum. 63:755.
2. Zakrzewska A, et al. 2010. Blood 116:e1.
3. Pham TH, et al. 2013. Nucleic Acids Res. 41:6391.
4. Pospisil V, et al. 2011. EMBO J. 30:4450.
5. Zarnegar MA, et al. 2010. Mol. Cell Biol. 30:4922.
6. Rimmele P, et al. 2010. Cancer Res. 70:6757.

Gene ID
20375 View all products for this Gene ID
UniProt
View information about SPI1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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