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Alexa Fluor® 594 anti-LIF Antibody

Pricing & Availability
Clone
M1506B09 (See other available formats)
Regulatory Status
RUO
Other Names
Leukemia inhibitory factor, HILDA (human interleukin in DA cells), MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor)
Isotype
Rat IgG2a, κ
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Product Citations
publications
M1506B09_AF594_LIF_Antibody_IF_073115
HeLa cells were fixed with ice cold methanol for five minutes and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/ml Alexa Fluor® 594 conjugated (red) LIF Antibody (clone M1506B09) in a blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • M1506B09_AF594_LIF_Antibody_IF_073115
    HeLa cells were fixed with ice cold methanol for five minutes and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/ml Alexa Fluor® 594 conjugated (red) LIF Antibody (clone M1506B09) in a blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
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674704 100 µg 268€
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Description

Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190, and the subunit gp130. The LIF complex receptor signals though STAT3 and JAK kinases. LIF expression has been observed in various tissues including the thymus, lung, and neuronal tissue. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contributes to stem cell self-renewal. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, as well as the induction of neuronal cell differentiation. It also regulates mesenchymal to epithelial conversion during kidney development, and also may have a role in immune tolerance at the maternal-fetal interface. LIF can be upregulated by pro-inflammatory cytokines such as TNF-α and IL-17. Elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse LIF produced in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2566178 (BioLegend Cat. No. 674704)

Antigen Details

Structure
202 amino acids with a predicted molecular weight of approximately 22 kD.
Distribution

Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.

Function
Inhibits embryonic stem cell differentation and induces the terminal differentiation of myeloid leukemia cells. LIF is upregulated by TNF-α and IL-17. LIF is downregulated by IL-4 and IL-13 in fibroblasts.
Interaction
Embryonic stem cells.
Ligand/Receptor
LIF receptor complex (gp190 and gp130).
Cell Type
T cells, Tregs
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Moreau JF, et al. 1988. Nature 336:690.
2. Tomida M, et al. FEBS Lett. 334:193.
3. Metcalfe SM. 2011. Genes Immun. 12:157.
4. Voyle RB, et al. 1999. Exp. Cell Res. 249:199.
5. Slaets H, et al. 2010. Trends Mol. Med. 16:493.
6. Souza PP, et al. 2012. Mol. Immunol. 49:601

Gene ID
3976 View all products for this Gene ID
UniProt
View information about LIF on UniProt.org
Go To Top Version: 2    Revision Date: 06/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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